摘要
目的 观察选择性环氧合酶 2抑制剂美罗昔康 (莫比可 )对单侧输尿管梗阻大鼠肾脏病变的影响。方法 将输尿管梗阻大鼠随机分为不用药组 (U)、消炎痛组 (I)和美罗昔康组 (M) ,设假手术组 (C)为对照。 4周后应用逆转录 聚合酶链式反应 (RT PCR)、免疫沉淀等方法分别检测转化生长因子 βⅠ型 (TβR 1)及Ⅱ型 (TβR 2 )受体的基因表达以及转化生长因子 β1(TGF β1)的蛋白水平 ,并观察肾小管间质纤维化病变。结果 与C组相比 ,TβR 1和TβR 2在U组大鼠左肾的表达显著上调 ,使用消炎痛、美罗昔康均能不同程度地抑制其含量 (P <0 0 1) ,尤以美罗昔康为著。TGF β1的蛋白水平被美罗昔康显著抑制 ,而消炎痛无效。U组出现了明显的肾小管间质纤维化 ,消炎痛使用后病变加重 ,而美罗昔康却能使之减轻 (P <0 0 1)。结论 美罗昔康能明显减轻肾小管间质的损害 ,TGF
Objective To observe the effect of selective cyclooxygenase 2(COX 2) inhibitor meloxicam on the progression of tubulointerstitial fibrosis in ratswith unilateral ureteral obstruction (UUO) Methods UUO rats were treated with meloxicam (M)?indomethacin (I) or vehicle alone (U) for 4 weeks Using reverse transcription polymerase chain reaction (RT PCR), we examined the mRNA expressions of transforming growth factor (TGF) β receptor 1 and 2 in left kidney from rats with UUO Immuno precipitation and immunohistochemistry analysis were carried out to investigate the protein level of TGF β 1 Tubulointerstitial fibrosis was quantified by Masson′s staining Similar studies were performed in another group of rats with sham operation (C) Results mRNA expressions of TGF β receptor 1 and 2 were markedly increased in Group U compared with those in Group C Both meloxicam and indomethacin inhibited their expressions to a different degree ( P <0 01), but the effect of meloxicam was more significant Meloxicam decreased the protein level of TGF β 1 ( P <0 01), while indomethacin had no such effect Compared with Group C, there appeared tubulointerstitial fibrosis in the kidney of rats with UUO The lesion was aggravated by indomethacin, but remarkably attenuated by meloxicam Conclusions In summary, selective COX 2 inhibitor meloxicam has a salutary effect on the tubular and interstitial response to UUO TGF β and its receptor approaches partly explain some of the mechanism
出处
《中华内科杂志》
CAS
CSCD
北大核心
2002年第12期825-828,共4页
Chinese Journal of Internal Medicine
基金
教育部博士点基金资助项目
