摘要
目的 观察光动力疗法对类风湿性关节炎模型病理及增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)表达的影响。方法 LEW大鼠用Ⅱ型胶原蛋白诱发关节炎 (CIA)。在大鼠出现踝关节红肿后1周 ,炎症达到高峰时进行光动力疗法 (PDT)治疗。一侧作为治疗组 ,另一侧作对照组。治疗方法是大鼠麻醉后每公斤体重尾静脉注入HMME 10mg ,2 0min后对踝关节进行激光照射 ,波长 62 7.8nm ,功率密度 10 0mW /cm2 ,能量密度 10 0J/cm2 。治疗后 2周 ,取关节进行病理及PCNA免疫组化染色观察。结果 PDT治疗组滑膜增生、血管翳形成及软骨破坏、骨破坏和总的病理评分比对照组好 ;治疗组PCNA阳性细胞减少 ,图像分析结果差异有显著性。结论 PDT能够减少类风湿性关节炎模型增生滑膜细胞的数量 ,减轻软骨和骨的破坏 ;
Objective To investigate the effect of photodynamic therapy (PDT) on histopathologic changes and proliferating cell nuclear antigen (PCNA) expression in the animal model of rheumatoid arthritis (RA) . Methods The rats with collageninduced arthritis (CIA) were injected intravenously with HMME at a dose of 10 mg/kg when the inflammatory reaction reached its peak in both ankles of these animals. Twenty minutes after the injection, one of the ankles was then randomly assigned to receive laser irradiation at a dose of 100 J/cm 2 for 1 000 s, while the contralateral ankle served as the control. The histopathologic changes of the ankle joint and the expression level of PCNA were assessed after two the weeks of the treatment. Results Histological examination showed statistically significant reductions in synovial hyperplasia , pannus formation and cartilage erosion as well as bone destruction in the PDT treated ankles than those in the contralateral side of these animals. The number of PCNA positive cells was obviously less than that in the control side. Image analysis also confirmed these beneficial effects of PDT. Conclusion PDT is useful for reducing synovial hyperplasia, cartilage erosion and bone destruction in CIA and helpful for reducing the PCNA positive cells .
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2002年第2期81-83,共3页
Chinese Journal of Physical Medicine and Rehabilitation
