摘要
目的 探讨不同浓度内皮素 1(ET 1)对早、晚期肝硬化大鼠离体肝脏及血管环调节作用及其变化规律。方法 皮下注射CCl4制备大鼠肝硬化模型 ,在造模 9周末和 14周末采用离体肝灌注和离体血管环技术研究不同浓度ET 1对早、晚期肝硬化大鼠肝血流动力学及血管环的调节作用。结果 晚期组 (n =8)肝硬化大鼠基础状态门静脉压 (Ppv) (2 36± 0 2 9)高于早期组 [1 5 4± 0 2 1,(n =8) ],均高于正常对照组 [1 18± 0 13,(n =8) ],各组间Phv差异无显著意义。给予ET 1后 ,门静脉灌注压 (PVP)随ET 1浓度增加而升高 ,晚期组较早期组升高更为明显 ,均高于正常对照组 ,而离体血管环对不同浓度ET 1的浓度累积反应曲线随病期进展而右移 ,同一病期血管环随ET 1浓度增加而收缩性增强 ,但均低于正常对照组 ,0 1nmol/LET 1对晚期组有轻度扩血管作用。结论 ET 1可明显增加肝循环阻力 ,在肝硬化大鼠此种阻力增加作用强于正常大鼠 ,且随肝硬化程度加重而增强 ,而外周血管对ET 1的反应性随病期进展而下降 ,表明ET 1在门脉高压的形成和维持中发挥重要作用。
Objective To investigate the effects of ET 1 on isolated perfused rat liver and vascular rings at early and late stages of cirrhosis Methods Liver cirrhosis was induced by an intraperitoneal injection of 50% CCl 4(0 3 ml/100g, twice a week) In the 9 th and 14 th weekend after injecting CCl 4, the isolated perfused liver and vascular rings were performed to evaluate effects of four concentrations of ET 1 on early and late stages of cirrhosis Results The Ppv of L HC group at baseline was higher than that of E HC group, both were higher than that of the controls However, there showed no differences on Phv in these groups With the concentration of ET 1 increasing, PVP was elevated accordingly in E HC and L HC group L HC group showed higher PVP compared with E HC group, both were higher than the controls While in isolated vascular rings, with the deteriorating of cirrhosis, the cumulative response curves showed right shift 0 1 nmol/L ET 1 showed mild relaxation on vascular rings in L HC group Conclusion ET 1 can increase the PVP, especially with the deterioration of cirrhosis, there showed higher reaction compared with normal controls The vascular rings showed low response on the contrary So ET 1 plays an important role in the pathogenesis of portal hypertension In view of its different roles on liver and vascular rings at early and late stages, administration of different selective antagonist of ET receptor at different stages of cirrhosis should be well considered
出处
《中华医学杂志》
CAS
CSCD
北大核心
2002年第22期1565-1568,共4页
National Medical Journal of China
