摘要
利用氧极谱法测定依赖乙醇酸和乙醛酸的氧吸收,利用分光光度计测定转氨酶活性的方法,检查了光呼吸碳代谢中各种中间产物对过氧化物酶体膜的透性。结果表明,O_2、乙醇酸、乙醛酸、丝氨酸、天冬氨酸、草酰乙酸、α-酮戊二酸能透过过氧体膜。过氧体膜可能有谷氨酸和 NADH 进入的屏障。过氧体悬浮液 Percoll 存在,很快使过氧体膜破坏。Percoll 也使羟基丙酮酸还原酶和苹果酸脱氢酶的活性很快降低。
Permeability of various metabolites in photorespiratory carbon metabolism pathway is ex- amined by O_2 electrode and spectrophotometry.The results show that O_2,glycolate,glyoxyla- te,serine,asparate and a-ketoglutarate are permeable to the peroxisomal membrane for matrix enzymes activity,and the peroxisomal membrane seems to be an entry barrier to glutamate and NADH.The integrity of peroxisomal membrane is disrupted rapidly by Percoll existing pero- xisome suspension.The activity of hydroxypyruvate reductase and malate dehydrogenase is also decreased by Percoll.
