摘要
目的:分析炎症性肠病(IBD)患者肠道菌群及粪便炎性标志物变化,以及不同疾病活动度患者的差异。方法:选择2022年3月至2024年2月于我院就诊的70例IBD患者作为研究组,其中溃疡性结肠炎(UC)40例,克罗恩病(CD)30例;选择同期接受结肠镜检查的健康体检者20人作为对照组。采集所有受试者新鲜粪便,进行细菌培养及炎性标志物粪便钙卫蛋白(FC)、乳铁蛋白(LF)、基质金属蛋白酶-9(MMP-9)、髓过氧化物酶(MPO)水平检测。比较不同活动度UC、CD患者及对照组受试者肠道菌群、粪便FC、LF、MMP-9、MPO水平的差异。结果:与对照组相比,IBD患者肠道中需氧菌之肠杆菌、肠球菌、酵母菌及厌氧菌之拟杆菌水平均明显升高(P<0.05),厌氧菌之双歧杆菌、乳杆菌、真杆菌水平均明显降低(P<0.05),需氧菌之葡萄球菌及厌氧菌之消化球菌和梭菌无明显变化(P>0.05)。与对照组相比,除UC患者厌氧菌之梭菌水平升高、而CD患者降低外(P<0.05),UC患者和CD患者其他菌群变化与IBD患者整体菌群变化一致。随疾病活动度增加,UC患者和CD患者需氧菌之肠杆菌、肠球菌、酵母菌及厌氧菌之拟杆菌水平依次升高(P<0.05),厌氧菌之双歧杆菌、乳杆菌、真杆菌水平依次降低(P<0.05)。与对照组相比,IBD患者粪便FC、LF、MMP-9、MPO水平显著升高(P<0.05),UC患者和CD患者粪便FC、LF、MMP-9、MPO水平变化与IBD患者整体变化一致。随疾病活动度增加,UC及CD患者粪便FC、LF、MMP-9、MPO水平依次增加(P<0.05)。结论:IBD患者肠道菌群及粪便炎性标志物水平发生显著变化,且不同疾病活动度患者间存在一定差异,有望为IBD活动度评估提供参考。
Objective To analyze the changes of intestinal flora and fecal inflammatory markers in patients with inflammatory bowel disease(IBD)and the differences of patients with different disease activity.Methods Total 70patients with IBD who were admitted to our hospital from March 2022to February 2024were selected as study group,including 40patients with ulcerative colitis(UC)and 30patients with Crohn's disease(CD).Twenty healthy subjects who received colonoscopy at the same time were selected as control group.Fresh feces of all subjects were collected,and bacterial culture and inflammatory markers of fecal calcarein(FC),lactoferrin(LF),matrix metalloproteinase-9(MMP-9)and myeloperoxidase(MPO)were detected.The intestinal flora,levels of FC,LF,MMP-9and MPO in the UC and CD patients with different activity levels and the subjects in the control group were compared.Results Compared with the control group,the levels of enterobacter,enterococcus,yeast of aerobic bacteria and bacteroides of anaerobic bacteria in the intestinal tract of IBD patients were significantly increased(P<0.05).The levels of bifidobacterium,lactobacillus and eubacterium of anaerobic bacteria were significantly decreased(P<0.05),and the levels of staphylococcus of aerobic bacteria,peptococcus and clostridium of anaerobic bacteria were not significantly changed(P>0.05).Compared with the control group,other flora changes in UC and CD patients were consistent with the overall flora changes in IBD patients,except that the level of clostridium of anaerobic bacteria was increased in UC patients and decreased in CD patients(P<0.05).With the increase of disease activity,the levels of enterobacter,enterococcus,yeast and bacteroides of aerobic bacteria in UC and CD patients were increased successively(P<0.05),while the levels of bifidobacterium,lactobacillus and eubacterium of anaerobic bacteria were decreased successively(P<0.05).Compared with the control group,the levels of FC,LF,MMP-9and MPO in IBD patients were significantly increased(P<0.05),and the changes of FC,LF,MMP-9and MPO in UC and CD patients were consistent with the overall changes in IBD patients.With the increase of disease activity,the levels of FC,LF,MMP-9and MPO in UC and CD patients were increased successively(P<0.05).Conclusion There are significant changes in intestinal flora and fecal inflammatory markers in patients with IBD,and there are some differences among patients with different disease activity,which is expected to provide reference for the assessment of IBD activity.
作者
于晶晶
胡选亚
邢旭
YU Jing-jing;HU Xuan-ya;XING Xu(Department of Clinical Laboratory,Nanyang First People's Hospital,Nanyang,Henan 473000)
出处
《中国肛肠病杂志》
2024年第11期45-48,共4页
Chinese Journal of Coloproctology
关键词
炎症性肠病
肠道菌群
粪便炎性标志物
疗效
Inflammatory bowel disease
Intestinal flora
Fecal inflammatory markers
Efficacy
