摘要
青枯病是桑树(Morus L.)的重大土传细菌性病害,其发生给蚕桑生产造成了严重的经济损失。通过转录组测序,探究桑树对青枯病病原菌Ralstonia pseudosolanacearum侵染的分子响应机制。通过采集广东省兴宁市罗岗镇发病桑树样品,并利用16S rDNA分子鉴定、多重PCR检测及序列变异分析等方法,结合系统发育分析和致病性试验,分别对侵染发病植株的根茎叶组织进行差异转录组分析,鉴定差异表达基因(differentially expressed genes,DEGs)。结果显示,Ⅲ型效应蛋白(typeⅢeffectors,T3Es)相关基因RipAW、RipAE及调控基因hrpB在侵染过程中高度表达,桑树体内防御相关基因SGT 1a、JAR 1a表达受到抑制,水通道蛋白基因MnPIP 1;1、MnTIP 4;1表达显著下调,糖运输相关基因SWEET 2、SWEET6 b表达上调。本研究为青枯病侵染桑树的分子响应机制研究提供了理论依据,并为制定有效的防控措施奠定了基础。
Mulberry bacterial wilt,caused by Ralstonia pseudosolanacearum,is a significant soilborne bacterial disease that impacts the mulberry industry,leading to considerable economic losses in sericulture.This study was conducted to explore the molecular response mechanisms of mulberry to R.pseudosolanacearum infection through transcriptome sequencing.Samples were collected from infected mulberry trees in Luogang Town,Xingning City,Guang-dong Province.Methods such as 16S rDNA molecular identification,multiplex PCR detection,sequence variation analysis,phylogenetic analysis,and pathogenicity tests were employed to analyze the differential transcriptome in the root,stem,and leaf tissues of the infected plants.This analysis identified various differentially expressed genes(DEGs).The results indicated that genes associated with typeⅢeffectors(T3Es),such as RipAW and RipAE,and the regulatory gene hrpB were highly expressed during infection.Conversely,the expression of defense-related genes such as SGT 1a and JAR 1a in mulberry was found to be suppressed,while the expression of water channel protein genes MnPIP 1;1 and MnTIP 4;1 was significantly downregulated.Additionally,the expression of sugar transport-related genes SWEET 2 and SWEET 6b was upregulated.These findings provide theoretical insights into the molecular response mechanisms of mulberry to R.pseudosolanacearum infection and establish a foundation for developing effective control strategies.
作者
刘梦圆
王思怡
代薛
史惠聪
李萍
吴福安
Liu Mengyuan;Wang Siyi;Dai Xue;Shi Huicong;Li Ping;Wu Fuan(Jiangsu Key Laboratory of Sericultural and Animal Biotechnology,School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212100,China;Key Laboratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture and Rural Affairs,Sericultural Scientific Research Center,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212100,China)
出处
《蚕业科学》
CAS
CSCD
北大核心
2024年第5期398-408,共11页
ACTA SERICOLOGICA SINICA
基金
江苏省自然科学基金项目(BK20210878)
江苏科技大学科研启动经费项目(1732932003)
江苏省现代农业重点研究发展计划项目(BE2021344)
江苏省研究生科研创新计划项目(KYCX23-3843)。
关键词
桑树
青枯病
转录组
致病性
差异表达基因
Mulberry
Bacterial wilt disease
Transcriptome
Pathogenicity
Differentially expressed genes
