摘要
胁迫记忆是植物抵御频发干旱胁迫的重要策略,目前桑树中干旱胁迫记忆的作用机制还不明确。以桑品种湖桑32号扦插苗为材料,进行单次干旱和往复干旱胁迫后分别对叶片进行RNA-seq分析。筛选到5749个单次干旱响应基因和6008个往复干旱响应基因,二者共有的3863个重叠基因即为潜在的干旱记忆基因(DMG)。GO和KEGG分析显示,DMG显著富集在ABA和JA生物合成及信号转导途径、氨基酸生物合成及转运途径、碳同化、果糖和麦芽糖代谢、光合作用、糖酵解、磷酸戊糖途径等途径。JA合成相关基因ACX 1和KAT 2及信号转导相关基因JAZ 8、ABA合成的2个NCED基因及其信号响应因子基因ARBP的表达量在往复干旱中不断上调;氨基酸外向转运蛋白基因表达量下调,内向转运蛋白基因表达量上调。光合电子传递相关基因PGR 5、热激蛋白基因HSP 17.6Ⅱ及转录因子基因bHLH 92、MYB 3也在单次干旱和往复干旱后呈现显著差异表达。研究结果为进一步解析桑树干旱记忆的转录调控机制提供了一定依据。
Stress memory is an important strategy for plants to defend against drought stress.However,the mechanisms of drought stress memory in mulberry are still unclear.In this study,saplings of mulberry variety Husang 32 propagated by cuttage were exposed to single and recurrent drought stresses and analyzed by RNA-seq technology.Totally 5749 single and 6008 recurrent drought-responsive genes were screened out.The 3863 genes in common were defined as potential drought memory genes(DMGs).GO and KEGG analyses showed that DMGs were significantly enriched in the ABA and JA biosynthesis and signaling path-ways,the amino acid biosynthesis and transport path-way,the carbon assimilation,fructose and maltose metabolism,photosynthesis,glycolysis,and pentose phosphate pathways.With the reoccurrence of drought,the expression of ACX 1 and KAT 2 that involved in JA biosynthesis and JAZ 8 related to JA signaling,and two NCED genes and ARBP related to ABA biosynthesis and signaling were constantly up-regulated.The expressions of several amino acid exporter genes were down-regulated,whereas the expression levels of amino acid importer genes were up-regulated.Photosynthetic electron transport-related gene PGR 5,heat shock protein gene HSP 17.6Ⅱand transcription factor genes bHLH 92 and MYB 3 also showed significantly different expressions after single drought and recurrent drought.The results provide a basis for further analysis of transcriptional regulation mechanism of drought memory in mulberry.
作者
方尧
顾晓晓
张文琪
翟泽洋
田岳
曹旭
刘利
Fang Yao;Gu Xiaoxiao;Zhang Wenqi;Zhai Zeyang;Tian Yue;Cao Xu;Liu Li(Jiangsu Key Laboratory of Sericultural and Animal Biotechnology,School of Biotechnology,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212100,China;Key Laboratory of Silkworm and Mulberry Genetic Improvement,Ministry of Agriculture and Rural Affairs,Sericultural Scientific Research Center,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212100,China)
出处
《蚕业科学》
CAS
CSCD
北大核心
2024年第5期385-397,共13页
ACTA SERICOLOGICA SINICA
基金
国家现代农业产业技术体系建设专项(CARS-18)
国家自然科学基金项目(31700527)。
关键词
桑树
干旱
胁迫记忆
转录组
Mulberry
Drought stress
Stress memory
Transcriptome
