摘要
本研究采用自制损伤装置制作不同损伤等级样本(DI组(撞击1次)和DII组(撞击不同位置2次)),利用低场核磁共振技术,在4℃和25℃两种贮存温度条件下,采集蓝莓损伤发生0、6、24 h的波谱信息和质子密度图像信息。结果表明,损伤显著改变了蓝莓内部水分状态与分布。随着时间的延长,受损蓝莓细胞壁水含量A21减少,而细胞质水含量A22在损伤发生后逐渐上升,特别在损伤程度高的DII组,损伤发生24 h后A22增至0 h的1.45倍以上,而液泡水含量A23和总水分含量A2大幅度下降,表明蓝莓损伤后水分重新分配和向外界环境迅速转移。随着损伤程度上升,液泡水主峰整体呈现显著下降和右移的趋势,同时温度的升高进一步加剧了该变化。在25℃条件下,损伤发生6 h和24 h时,出现了4个弛豫峰,弛豫峰的增加标志着蓝莓品质显著劣变。相比于室温(25℃),4℃低温贮存有利于保持果实的完整性,并减缓受损蓝莓水分流失速率。实验结果可为实现蓝莓机械损伤的快速检测和损伤机理研究提供理论基础和数据参考。
In this study,a custom apparatus was utilized to obtain blueberry samples with high and low levels of mechanical injury(DI and DII group),which were subsequently stored at 4 or 25℃and analyzed using low-field nuclear magnetic resonance(LF-NMR)spectroscopy.Spectroscopic data and proton density images were collected at 0,6,and 24 h post-damage.The results demonstrated a significant impact of injury on the water state and distribution of blueberries.Notably,the cell wall water content A21 decreased,whereas the cytoplasm water content A22 increased over time post-damage.The A22 of the high injury group increased by 1.45 times at 24 h compared with that at 0 h,while the vacuole water content A23 and the total water content A2 notably declined,indicating a rapid redistribution and transfer of water from damaged blueberries to the external environment.As the degree of damage increased,the major peak of vacuole water shifted to the right and decreased significantly.Furthermore,increasing storage temperature further exacerbated these changes.At 25℃,the emergence of 4 relaxation peaks at both 6 and 24 h after damage indicated a significant deterioration in the quality of blueberries.Compared with 25℃,storage at 4℃was more conducive to maintaining fruit integrity and slowing down the rate of moisture loss from damaged blueberries.The experimental results provide a theoretical basis and data reference for the rapid detection and mechanistic understanding of mechanical damage in blueberries.
作者
罗子旋
姜凤利
吴沛净
宋平
丁柯柯
LUO Zixuan;JIANG Fengli;WU Peijing;SONG Ping;DING Keke(College of Information and Electrical Engineering,Shenyang Agricultural University,Shenyang 110866,China)
出处
《食品科学》
EI
CAS
北大核心
2025年第3期204-211,共8页
Food Science
基金
辽宁省科技厅揭榜挂帅课题项目(2022JH1/10900006)
辽宁省教育厅基础研究项目(LJKMZ20221033)。
关键词
蓝莓
机械损伤
温度
低场核磁共振
水分分布
blueberry
mechanical injury
temperature
low-field nuclear magnetic resonance spectroscopy
moisture distribution
