摘要
建立了猪肉样品中痕量氨基糖苷类抗生素(Aminoglycosides,AGs)(阿米卡星、安普霉素、潮霉素B、卡那霉素、巴龙霉素、核糖霉素、大观霉素、链霉素、妥布霉素)残留的检测方法。猪肉样品经过冷冻干燥、研磨后,加入150 mmol/L EDTA溶液和5%TCA溶液进行超声提取,经Oasis HLB柱净化后,采用BEH Z-HILIC色谱柱,进行超高效液相色谱-串联质谱分析。9种AGs在0.01~5μg/mL范围内线性关系良好,相关系数(R^(2))均大于0.999。猪肉样品中AGs的定量限为0.01~0.29μg/g。在低、中、高3种加标水平下,猪肉样品中AGs的平均加标回收率为79.91%~113.20%,相对标准偏差为1.26%~12.40%。该方法操作简单、灵敏度高,适用于猪肉中AGs残留的检测。
The method was developed for the determination of trace aminoglycoside antibiotics(AGs)(Amikacin,Apramycin,Hygromycin B,Kanamycin,Paromomycin,Ribostamycin,Spectinomycin,Streptomycin,Tobramycin)residues in pork sample.The pork samples were freeze-dried and ground,then ultrasonically extracted by adding 150 mmol/L EDTA solution and 5%TCA solution,cleaned up by an Oasis HLB column,and then analyzed by ultra-performance liquid chromatography-tandem mass spectrometry.The linear relationships of the 9 AGs were in a good range of 0.01~5μg/mL(R^(2)≥0.999).The limits of quantitation were in the range of 0.01~0.29μg/g.At the three low,medium,and high spiking levels,the average spiking recovery rates of AGs in pork samples ranged from 79.91% to 113.20%,with relative standard deviations of 1.26%~12.40%.This method is simple,sensitive,and suitable for detecting of AGs residues in pork.
作者
周寒琳
郭杭婷
张虹
陈新旺
韩永和
张勇
贲玉婕
ZHOU Han-lin;GUO Hang-ting;ZHANG Hong;CHEN Xin-wang;HAN Yong-he;ZHANG Yong;BEN Yu-jie(College of Environmental and Resource Science,College of Carbon Neutral Modern Industry,Fujian Normal University,Fuzhou 350117,China;Fujian Key Laboratory of Pollution Control and Resource Reuse,Fujian Normal University,Fuzhou 350117,China;Department of Pulmonary and Critical Care Medicine,Fujian Medical University Union Hospital,Fuzhou 350001,China;Eastern Institute of Technology,Ningbo,Ningbo 315200,China)
出处
《化学试剂》
CAS
2025年第1期86-91,共6页
Chemical Reagents
基金
福建省自然科学基金面上项目(2022J01184)。
关键词
氨基糖苷类抗生素
固相萃取
超高效液相色谱-串联质谱法
猪肉
aminoglycoside antibiotics
solid phase extraction
ultra-performance liquid chromatography-tandem mass spectrometry
pork
