摘要
利用同位素稀释技术建立一种可同时测定生活饮用水中二氯乙酸、三氯乙酸、氯酸盐、亚氯酸盐和溴酸盐5种消毒副产物以及高氯酸盐的超高效液相色谱串联质谱(ID-UPLC-MS/MS)检测方法。样品过膜后加入同位素内标,采用Torus DEA色谱柱(100 mm×2.1 mm,1.7μm)分离,以0.5%氨水-1 mmol/L乙酸铵和乙腈为流动相洗脱,多反应监测(MRM)负模式(ESI-)测定,内标法定量。6种化合物线性相关系数r均大于0.990,检出限为1.5~2.1μg/L,定量限为5.0~7.0μg/L,应用该方法对399份水样进行测定,检出率为16.04%。该方法采用同位素内标稀释直接进样,分析速度快,灵敏度高,数据准确适用于饮用水国家标准(GB/T 5750.5—2023和GB/T 5750.10—2023)检测限值的要求。
An isotope dilution technique coupled with ultra-performance liquid chromatography-tandem mass spectrometry(ID-UPLC-MS/MS)method was established for simultaneous determination of disinfection byproducts such as dichloroacetic acid,trichloroacetic acid,chlorate,chlorite,bromate,and perchlorate in drinking water.The samples were separated by Torus DEA column(100 mm×2.1 mm,1.7μm),eluted with 0.5% ammonia-1 mmol/L ammonium acetate and acetonitrile as mobile phases,determined by multiple reaction monitoring(MRM)negative mode(ESI-),and quantified by internal standard method.The linear correlation coefficients for all six compounds were greater than 0.990.The detection limits was 1.5~2.1μg/L,and the quantitation limits was 5.0~7.0μg/L.The detection rate was 16.04% in 399 water samples.The method involves direct injection of samples using isotope internal standard dilution,resulting in fast analysis speed,high sensitivity,and accurate data.This method is suitable for detecting compounds within the limits set by the drinking water national standards(GB/T 5750.5—2023 and GB/T 5750.10—2023).
作者
侯婧
刘双德
HOU Jing;LIU Shuang-de(Qinghai Center for Disease Prevention and Control,Xining 810000,China)
出处
《化学试剂》
CAS
2025年第1期67-73,共7页
Chemical Reagents
关键词
生活饮用水
超高效液相色谱串联质谱法
消毒副产物
高氯酸盐
Torus
DEA色谱柱
同位素内标稀释
drinking water
ultra performance liquid chromatography-tandem mass spectrometry
disinfection byproducts
perchlorate
torus DEA column
isotope internal standard dilution
