摘要
二维金属-有机框架材(2D-MOFs)具有大比表面积、厚度小、活性位点充分暴露、传质阻力小等优点,将其用作高效液相色谱(HPLC)固定相理论上可以增加色谱柱的塔板数,进而提高其分离效能,在手性分离领域展现出独特的应用价值。将手性分子L-羟基脯氨酸和D-(+)-葡萄糖分别修饰到NH_(2)-Ni/Co MOFs和Zn-NH_(2)-BDC MOFs两种2D-MOFs上,制备二维手性金属-有机框架纳米片(2D-CMOFs),再将Zn-NH_(2)-BDC-D-(+)-葡萄糖和NH_(2)-Ni/Co-L-羟基脯氨酸通过“网包法”固载在硅胶表面制备手性固定相。实验表明:19种手性药物和5种位置异构体被NH_(2)-Ni/Co-L-羟基脯氨酸手性色谱柱拆分开,基线分离了6种手性药物和3种位置异构体;10种手性药物和3种位置异构体被Zn-NH_(2)-BDC-D-(+)-葡萄糖色手性谱柱拆分开;且两个手性柱都具有良好的重复性。
Two-dimensional metal-organic frame materials(2D-MOFs)have the advantages of large specific surface area,small thickness,full exposure of active sites,and minimal mass transfer resistance.Their application in high-performance liquid chromatography(HPLC)can theoretically increase the number of column plates and improve its separation efficiency,showing unique application value in the chiral separation field.Two-dimensional chiral metal-organic framework nanosheets(2D-CMOFs)were prepared by modifying the chiral molecules L-hydroxyproline and D-(+)-glucose onto NH_(2)-Ni/Co MOFs and Zn-NH_(2)-BDC MOFs,respectively.The chiral stationary phase was created by applying NH_(2)-Ni/Co-L-hydroxyproline and Zn-NH_(2)-BDC-D-(+)-glucose onto the silica gel surface using the“mesh coating method”.The results showed that 19 chiral drugs and 5 site isomers were separated by NH_(2)-Ni/Co-L-hydroxyproline chiral column,and 6 chiral drugs and 3 site isomers were baseline separated.Ten chiral drugs and three site isomers were separated by Zn-NH_(2)-BDC-D-(+)-glucose-colored chiral spectrum.Both chiral columns have good repeatability.
作者
王雪萍
高顺秋
李毅洁
杨梅芳
龙怡幸
袁黎明
WANG Xue-ping;GAO Shun-qiu;LI Yi-jie;YANG Mei-fang;LONG Yi-xing;YUAN Li-ming(School of Chemistry and Chemical Engineering,Yunnan Normal University,Kunming 650500,China)
出处
《化学试剂》
CAS
2025年第1期17-25,共9页
Chemical Reagents
基金
基于二维材料的薄片型气相色谱柱手性柱研究项目(22174125)。
关键词
手性分离
高效液相色谱
二维手性金属-有机框架材料
手性固定相
手性化合物
chiral separation
high-performance liquid chromatography
two-dimensional chiral metal-organic frameworks
chiral stationary phase
chiral compounds
