摘要
[目的]通过对喂养芬荠素小鼠脾的代谢组学分析,探讨芬荠素对小鼠脾组织代谢的影响。[方法]健康雄性SPF级C57小鼠16只,分为芬荠素处理组、对照组,经过8 w自由进食喂养后,取小鼠脾脏进行处理检测,运用高效液相色谱质谱联用仪检测,找出具有差异的特征代谢物及差异代谢通路。[结果]经代谢组学分析,与对照组相比,处理组共筛选出15种差异代谢物,显著影响了6条代谢通路,包括组氨酸代谢、鞘脂代谢、甘油磷脂代谢、D-谷氨酰胺和D-谷氨酸代谢、精氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢等代谢通路。[结论]芬荠素干扰了小鼠体内多条代谢通路的平衡,降低了小鼠的抗炎症和抗氧化能力,对小鼠的机体健康造成了损伤。
[Objective]The effects of fengycin on the metabolism of mouse spleen tissues were investigated by metabolomic a-nalysis of the spleens of fengycin-fed mice.[Method]Sixteen healthy male SPF-grade C57 mice were divided into the fengy-cin-treated group and the control group.After eight weeks of ad libitum feeding,the spleens of the mice were processed and detected by high performance liquid chromatography-mass spectrometry to identify the characteristic metabolites and metabolic pathways with differences.[Result]After metabolomics analysis,15 different metabolites were identified in the treated group compared with the control group,which significantly affected six metabolic pathways,including histidine metabolism,sphingolip-id metabolism,glycerophospholipid metabolism,D-glutamine and D-glutamate metabolism,arginine metabolism,and alanine,aspartic acid and glutamate metabolism.[Conclusion]Fengycin interfered with the homeostasis of multiple metabolic pathways in mice and reduced their anti-inflammatory and antioxidant capacity,causing damage to their organic health.
作者
徐海峰
刘青
张阳
郑雨佳
李宇健
贺佳诺
王一凡
米婧璇
赵雯雅
杨雪
赵山山
郝光飞
刘洪伟
XU Haifeng;LIU Qing;ZHANG Yang;ZHENG Yujia;LI Yujian;HE Jianuo;WANG Yifan;MI Jingxuan;ZHAO Wenya;YANG Xue;ZHAO Shanshan;HAO Guangfei;LIU Hongwei(Institute of Biology,Hebei Academy of Sciences,Shijiazhuang 050081;College of Life Sciences and Food Engineering,Hebei Engineering University,Handan 056038,China)
出处
《生物技术》
CAS
2024年第6期715-721,共7页
Biotechnology
基金
河北省科学院科技计划项目(24308)
河北省科学院高层次人才培养与资助项目(2024G11)
河北省重点研发计划项目(23372401D)。
