摘要
研究不同浓度梯度外源褪黑素(Melatonin,MT)处理对盐胁迫下包瓜种子萌发和幼苗生长的影响,以寻找促进包瓜种子萌发和对幼苗耐盐缓解效应最适宜的外源褪黑素浓度。试验材料为甜瓜品种包瓜。种子试验以0μmol/L MT(T0)、50μmol/L MT(T1)、100μmol/L MT(T2)、200μmol/L MT(T3)、300μmol/L MT(T4)进行浸种处理,180 mmol/L NaCl溶液作为发芽溶液来模拟盐胁迫环境,设置完全空白对照组(CK,0μmol/L MT+0 mmol/L NaCl)。幼苗试验采用穴盘栽培,以浇灌180 mmol/L NaCl模拟盐胁迫环境,以喷施0μmol/L MT(MT0)、100μmol/L MT(MT100)为处理组,设置完全空白对照组(CK,0μmol/L MT+0 mmol/L NaCl)。通过测定分析各处理组生长及生理生化指标讨论各处理组的耐盐缓解效应。盐胁迫导致包瓜种子发芽势、发芽率、发芽指数等指标大幅度降低,幼苗叶片的叶绿素含量、叶长、叶宽、POD活性、CAT活性等指标均有所降低,而丙二醛(MDA)含量则有所升高。对盐胁迫下种子提前进行外源褪黑素浸种,并喷施外源褪黑素处理盐害幼苗后,各项指标都有所缓解,T2处理较T 0组显著促进种子萌发相关指标,MT100处理较MT0处理叶绿素含量增加30.92%,POD活性和CAT活性分别增加了40.22%和26.24%,MDA含量减少26.23%。对比各处理组检测结果发现,100μmol/L MT促进包瓜种子萌发的效果最好,且对盐害幼苗耐盐缓解效应显著。
The aim of studying the effects of different concentrations of exogenous melatonin treatment on the germination and seedling growth of balloon cucumber under salt stress is to find the most suitable concentration of exogenous melatonin that can promote the germination of balloon cucumber seeds and alleviate the salt tolerance of seedlings.The experimental material is the melon variety Baogua.For the seed experiment,seeds were soaked in different concentrations of melatonin(MT)including 0μmol/L MT(T0),50μmol/L MT(T1),100μmol/L MT(T2),200μmol/L MT(T3),and 300μmol/L MT(T4).A 180 mmol·L-1 NaCl solution was used as the germination solution to simulate a salt stress environment.A complete blank control group(CK,0μmol/L MT+0 mmol/L NaCl)was also set up.For the seedling experiment,a plug tray cultivation method was adopted.Seedlings were subjected to salt stress by watering with 180 mmol/L NaCl,and sprayed with 0μmol/L MT(MT0)and 100μmol/L MT(MT100)as the treatment groups.A complete blank control group(CK,0μmol/L MT+0 mmol/L NaCl)was also set up.The salt tolerance alleviation effects of each treatment group were discussed by measuring and analyzing the growth,physiological,and biochemical indicators of each treatment group.The experimental results showed that salt stress significantly reduced the germination potential,germination rate,germination index,and other indicators of'Baogua'seeds.Similarly,the chlorophyll content,leaf length,leaf width,POD activity,and CAT activity of the seedling leaves were also reduced,while the malondialdehyde(MDA)content increased.After pre-treating the seeds with exogenous melatonin soaking and spraying exogenous melatonin on salt-stressed seedlings,all indicators showed some degree of alleviation.Among them,the T2 treatment significantly promoted seed germination-related indicators compared to the T0 group.The MT100 treatment group showed 30.92%increase in chlorophyll content compared to the MT0 treatment group,40.22%and 26.24%increase in POD and CAT activities respectively,and 26.23%reduction in MDA content.The comparison of the test results from various treatment groups indicated that 100μmol/L MT was the optimal concentration for promoting germination of Baogua melon seeds,and it had a significant effect on alleviating salt stress in seedlings suffering from salt injury.
作者
刘春
徐向锋
王晨阳
杨德堃
杨婧
杨星月
李锦锦
LIU Chun;XU Xiangfeng;WANG Chenyang;YANG Dekun;YANG Jing;YANG Xingyue;LI Jinjin(School of Life Sciences,Huaibei Normal University,Huaibei 235000,China;Library,Huaibei Normal University,Huaibei 235000,China)
出处
《安徽科技学院学报》
2025年第1期17-25,共9页
Journal of Anhui Science and Technology University
基金
安徽省西瓜甜瓜生物育种研究工程中心开放基金(AHXTKF2023003)
淮北师范大学校级重点实验室开放项目(2023syskf018)
2023年淮北师范大学校级教研重大项目(2023jxyj002)
淮北师范大学校级一流课程建设项目“特种果树学”(2023ylkc003)
安徽省质量工程项目(2022zybj085,2023sx068)。
关键词
甜瓜
外源褪黑激素
盐胁迫
种子萌发
幼苗
melon
exogenous melatonin
salt stress
seed germination
seedling growth
