摘要
目的初步了解新型负性分子Siglec-9在肺结核中的变化特点和作用。方法利用R语言对GSE83456芯片数据进行生物信息学分析。纳入确诊肺结核(PTB)患者48例,健康对照组46例,采用实时荧光定量PCR(qRT-PCR)检测外周血单个核细胞(PBMC)Siglec-9 mRNA表达量;采用流式细胞术检测Siglec-9^(+)CD4^(+)T细胞比例;采用CBA法检测TNF-α、IL-6、IFN-γ、IL-4水平;分析Siglec-9^(+)CD4^(+)T细胞比例与TNF-α等细胞因子水平相关性。结果生物信息学分析显示Siglec-9在PTB患者中明显升高(P<0.001),与TNF-α/NF-κB、IL-6/JAK/STATA3、PI3K/AKT/mTOR信号通路相关;实验结果显示Siglec-9^(+)CD4^(+)T细胞比例在PTB患者中明显升高(P<0.001)且与TNF-α等细胞因子水平均呈负相关。结论在PTB患者中,Siglec-9mRNA和Siglec-9^(+)CD4^(+)T细胞水平升高,可能介导对CD4^(+)T细胞的抑制作用,参与PTB的发生和发展。
This study was performed to investigate the variation characteristics and functions of Siglec-9 immune checkpoint in pulmonary tuberculosis.R language was used for bioinformatics analysis of GSE83456 chip data.Total of 48 patients with confirmed pulmonary tuberculosis(PTB)and 46 healthy controls were included.Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the mRNA expression of Siglec-9 in peripheral blood;flow cytometry was used to detect the proportion of Siglec-9^(+)CD4^(+)T cells.The levels of TNF-α,IL-6,IFN-γand IL-4 were detected by Cytometric Bead Array(CBA).Furthermore,the correlation of the proportion of Siglec-9^(+)CD4^(+)T cells with TNF-αand other cytokines were analyzed.Bioinformatics analysis showed that Siglec-9 was significantly increased in patients with PTB(P<0.001),and was related to TNF-α/NF-κB,IL-6/JAK/STATA3,PI3K/AKT/mTOR signal pathways.Experimental data showed that the proportion of Siglec-9^(+)CD4^(+)T cells was significantly increased in patients with PTB(P<0.001)and negatively correlated with the levels of TNF-αand other cytokines.In conclusion,the higher levels of Siglec-9 mRNA and Siglec-9^(+)CD4^(+)T cells in PTB may inhibit the function of CD4^(+)T cells and participate in the occurrence and development of PTB.
作者
何月月
朱玥洁
殷郑伟
史娟
商凯钰
田婷婷
史绘东
丁剑冰
张峰波
HE Yueyue;ZHU Yuejie;YIN Zhengwei;SHI Juan;SHANG Kaiyu;TIAN Tingting;SHI Huidong;DING Jianbing;ZHANG Fengbo(State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia,School of Basic Medicine Sciences,Xinjiang Medical University,Urumq 830011,China;Clinical Laboratory Center and Reproductive Assistance Center,First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China)
出处
《免疫学杂志》
CAS
CSCD
2024年第6期526-532,共7页
Immunological Journal
基金
省部共建中亚高发病成因与防治国家重点实验室开放课题(SKL-HIDCA-2021-JH11)
自治区科技援疆计划(2022E02061)。
