摘要
目的探究石墨烯远红外对顺铂化疗后的Lewis肺癌荷瘤小鼠相关免疫指标的影响。方法建立Lewis肺癌荷瘤小鼠免疫损伤模型,将小鼠分为模型对照组(CTL)、单独化疗组(DDP)、石墨烯低功率组(G-Low,30℃)、石墨烯高功率组(G-High,35℃)。检测小鼠的肿瘤体积增殖率、胸腺指数和脾脏指数、脾脏淋巴细胞增殖活性及NK细胞杀伤活性、外周血中白细胞数量及CD4^(+)T/CD8^(+)T的比值。结果与CTL组相比,DDP组小鼠胸腺指数、脾脏指数明显降低(P<0.01);与DDP组相比,G-Low组小鼠肿瘤抑制率显著提升(P<0.05),G-High组小鼠肿瘤抑制率极显著提高(P<0.01)。G-High组小鼠胸腺指数、白细胞数量、外周血CD4^(+)T/CD8^(+)T比值、脾脏NK细胞的杀伤活性均显著高于DDP组(P<0.05)。结论石墨烯远红外对顺铂化疗造成的Lewis肺癌荷瘤小鼠的免疫损伤具有良好的修复作用,且能显著抑制瘤体的生长。
This study was designed to investigate the effect of graphene far-infrared irradiation on the relevant immune indexes of Lewis lung cancer tumor bearing mice after cisplatin chemotherapy.A lung cancer tumor-bearing mouse immune damage model was established in Lewis mice,and the model mice were divided into a model control group(CTL),a chemotherapy group(DDP),a low-power graphene group(G-Low,30℃),and a high-power graphene group(G-High,35℃).Mice in all groups were subjected to detect the tumor volume growth rate,thymus index and spleen index,spleen lymphocyte proliferation activity and NK cell killing activity,the number of white blood cells in the peripheral blood and the ratio of CD4^(+)T/CD8^(+)T in the peripheral blood.Compared with the CTL group,the thymus index and spleen index of the DDP group were significantly lower(P<0.01);compared with the DDP group,the tumor inhibition rate of the G-Low group was significantly improved(P<0.05),and the tumor inhibition rate of the G-High group was extremely significantly improved(P<0.01).The thymus index,white blood cell count,peripheral blood CD4^(+)T/CD8^(+)T ratio,and NK cell killing activity of the spleen in the G-High group were significantly higher than those in the DDP group(P<0.05).Taken together,Graphene far-infrared irradiation has a good repair effect on the immune damage caused by cisplatin chemotherapy in Lewis lung cancer tumorbearing mice,and can significantly inhibit tumor growth.
作者
汪晨
范永超
穆祥
赵子涵
范梦雨
任格
冯波
张倩
WANG Chen;FAN Yongchao;MU Xiang;ZHAO Zihan;FAN Mengyu;REN Ge;FENG Bo;ZHANG Qian(Beijing Key Laboratory of Traditional Chinese Veterinary Medicine,Animal Science and Technology College,Beijing University of Agriculture,Beijing 102206,China;Graphene Medical Research Institute,Shandong University of Traditional Chinese Medicine,Ji’nan 250355,China)
出处
《免疫学杂志》
CAS
CSCD
2024年第6期520-525,共6页
Immunological Journal
基金
北京市教育委员会科技计划(KM202210020004)
国家自然科学基金(32202852)
市属高校分类发展-都市农林特色教师队伍建设(11000024T000002961733)。
关键词
石墨烯
荷瘤小鼠
抑瘤率
免疫抑制
免疫修复
Graphene
Tumor bearing mice
Tumor suppression rate
Immunosuppression
Immune repair
