摘要
番茄颈腐根腐病是近年来极具破坏性的番茄土传病害之一,严重影响番茄的品质和产量。为挖掘番茄颈腐根腐病抗性相关基因,本研究利用全基因关联分析(GWAS)和遗传定位对番茄颈腐根腐病抗性基因进行研究。首先对来自国内外的200份番茄材料进行全基因组关联分析,在番茄9号染色体定位到抗颈腐根腐病的位点,再利用抗病材料KP13及感病材料T15196的双亲群体进行连锁作图,综合GWAS和连锁作图结果,将Frl定位于标记SSR105和FrlSNP20之间,遗传距离约为0.8 cM,物理距离约为93 kb。筛选出4个属于番茄谷胱甘肽硫转移酶(SlGST)家族成员的候选基因Solyc09g011510.2.1、Solyc09g011580.2.1、Solyc09g011600.2.1和Solyc09g011630.2.1。获得1个与抗病性状紧密连锁的标记FrlSNP25,利用该标记对94个番茄材料进行抗性鉴定,样品基因型与颈腐根腐病抗性鉴定表型吻合率92.55%。本研究为番茄抗颈腐根腐病种质创新和揭示番茄抗颈腐根腐病抗性机理奠定了基础。
Fusarium crown and root rot(FCRR)of tomato(Solanum esculentum)is one of the most damaging soil-borne diseases affecting tomatoes in recent year,significantly impacting both the quality and yield of tomato.To explore candidate genes associated with resistance to FCRR in tomatoes,a genome-wide association study(GWAS)combined with genetic mapping was used.A total of 200 tomato materials from around the world were used for the GWAS,leading to the identification of a major locus associated with the resistance to FCRR on tomato chromosome 9.A genetic linkage map was constructed using a parental population derived from a cross between the resistant line KP13 and the susceptible line T15196.Based on the results of the GWAS and linkage mapping,the resistance locus(Frl)was mapped between the markers FrlSNP20 and SSR105,with a genetic distance of approximately 0.8 cM and a physical distance of about 93 kb.Four candidate genes belonging to the tomato glutathione S-transferase(SlGST)gene family were identified,including Solyc09g011510.2.1,Solyc09g011580.2.1,Solyc09g011600.2.1 and Solyc09g011630.2.1.The FrlSNP25 marker,which is tightly linked to the resistance of FCRR,was obtained and used to identify the resistance of 94 tomato accessions.The concordance rate between genotypic and phenotypic resistance was 92.55%for Fusarium crown and root rot.This study lays a foundation for the innovation of tomato germplasm and the understanding of resistance mechanisms in tomato against Fusarium crown and root rot.
作者
叶青静
王荣青
姚祝平
阮美颖
周国治
万红建
李志邈
程远
YE Qingjing;WANG Rongqing;YAO Zhuping;RUAN Meiying;ZHOU Guozhi;WAN Hongjian;LI Zhimiao;CHENG Yuan(Institute of Vegetables,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021)
出处
《核农学报》
CAS
北大核心
2025年第2期244-254,共11页
Journal of Nuclear Agricultural Sciences
基金
浙江省科技特派员项目(2020R23A58D03)
浙江省自然科学基金(LY19C150010)
浙江省院地合作项目(2023R23T77C02)。
关键词
番茄
颈腐根腐病
基因定位
抗性鉴定
tomato
Fusarium crown and root rot
gene mapping
resistance identification
