摘要
以大叶相思苗木为试验材料,利用VA菌根接种与外源钙施用的方法,探究了外源钙对VA菌根接种及干旱胁迫下VA菌根接种与外源钙对苗生长生理方面的影响。结果表明:在0~100 g/盆CaCl_(2)施用量中,以50 g/盆(45 mmol·L^(-1))CaCl_(2)处理下的菌根侵染率最高(61.2%)。相较75%土壤含水量,40%土壤含水量导致苗木高、径生长增量减少,叶片相对水分亏缺(RWD)增加,叶绿素含量、净光合速率(Pn)、水分利用效率(WUE)、胞间二氧化碳浓度(Ci)、超氧化物歧化酶(SOD)活性、可溶性糖含量降低,蒸腾速率(Tr)、气孔导度(Gs)、丙二醛(MDA)含量升高,干旱胁迫伤害明显。干旱胁迫下,VA菌根接种、施钙处理均显著改善了苗木生长生理表现,其中以VA菌根接种+外源钙复合处理下的苗木高径生长增量最大。以苗高、地径增量及RWD、叶绿素含量、Pn、Tr、WUE、Gs、Ci、MDA、SOD、可溶性糖含量12个指标为评价指数,通过主成分分析进行苗木抗旱能力综合评价,结果表现为:单一性菌根处理下苗木抗旱能力最强,其次是菌根与外源钙复合处理。研究认为,外源钙有利于大叶相思苗木VA菌根接种,菌根化苗的抗旱能力明显改善,但钙施用量应控制在适度范围。
Using Acacia auriculiformis seedlings as experimental materials,the effects of exogenous calcium on mycorrhizal inoculation and the combined effects of mycorrhizal inoculation and exogenous calcium on seedling growth and physiology under drought stress were investigated through mycorrhizal inoculation and exogenous calcium application.The results showed that among the CaCl_(2)application rates ranging from 0 to 100 g/pot,the highest mycorrhizal infection rate(61.2%)was observed with 50 g/pot(45 mmol·L^(-1))of CaCl_(2).Compared to 75%soil water content,40%soil water content resulted in decreased height and diameter growth increments of the seedlings,increased relative water deficit(RWD)in leaves,decreased chlorophyll content,net photosynthetic rate(Pn),water use efficiency(WUE),intercellular CO_(2)concentration(Ci),superoxide dismutase(SOD)activity,and soluble sugar content,and increased transpiration rate(Tr),stomatal conductance(Gs),and malondialdehyde(MDA)content,indicating significant drought stress damage.Under drought stress,both mycorrhizal inoculation and calcium application significantly improved the growth and physiological performance of the seedlings,with the combined treatment of mycorrhizal inoculation and exogenous calcium resulting in the greatest increase in height and diameter growth.Using 12 indicators including seedling height and ground diameter increments,RWD,chlorophyll content,Pn,Tr,WUE,Gs,Ci,MDA,SOD,and soluble sugar content as evaluation indices,a comprehensive evaluation of seedling drought resistance was conducted through principal component analysis.The results showed that seedlings treated solely with mycorrhizal inoculation had the strongest drought resistance,followed by those treated with a combination of mycorrhizal inoculation and exogenous calcium.The study suggests that exogenous calcium is beneficial for mycorrhizal inoculation in A.auriculiformis seedlings,and the drought resistance of mycorrhizal seedlings is significantly improved,but the amount of calcium application should be controlled within a moderate range.
作者
黄亦雄
HUANG Yi-xiong(Qingxin District Ecological Forest Management Center,Qingyuan,Guangdong 511810)
出处
《陕西林业科技》
2024年第6期13-18,共6页
Shaanxi Forest Science and Technology
关键词
大叶相思
菌根
钙
抗旱性
生长生理
Acacia auriculaeformis
mycorrhiza
calcium
drought resistance
growth and physiology
