摘要
植物生长调节因子(growth-regulating factor,GRF)是一类植物特异性转录因子,在植物的生长发育和胁迫响应中发挥着重要作用。本研究利用生物信息学,从蓝莓(Vaccinium darrowii)中鉴定出12个GRF家族成员,其中一个成员(Vadar_g32550)与拟南芥(Arabidopsis thaliana)AtGRF3和AtGRF4同源,我们将其命名为VdGRF5。该基因编码序列(CDS)长1242 bp,编码413个氨基酸,位于蓝莓1号染色体上,为亲水蛋白,没有跨膜结构,含有与植物生长发育、应激响应和生物与非生物胁迫响应相关的顺式作用元件。定量分析表明VdGRF5基因在幼叶、幼茎和小果中表达量较高,会被高盐胁迫明显诱导而上调表达。构建过表达载体,异源表达VdGRF5使拟南芥叶子面积变大、根增长,并增强了其耐盐性。本文为研究VdGRFs在影响蓝莓生长和抗盐胁迫中的作用提供参考,并确定了一个调控蓝莓发育和抗逆境育种的候选GRF基因。
Plant growth-regulatory factors(GRFs)are a class of plant-specific transcription factors that play important roles in plant growth and development and stress response.In this study,12 GRF family members from blueberry(Vaccinium darrowi)were identified by using bioinformatics.One of them(Vadar.g32550)is homologous to Arabidopsis thaliana GRF3 and GRF4,and named VdGRF5.The coding sequence(CDS)of VdGRF5 is 1242 bp long,encoding 413 amino acids,and it is located on blueberry chromosome 1.VdGRF5 is a hydrophilic protein without a transmembrane structure,and contains cis-acting elements related to plant growth and development,hormone response,and biotic and abiotic stress response.Quantitative analysis showed that the VdGRF5 gene was highly expressed in young leaves,young stems and small fruits,and significantly induced and up-regulated by high salt stress.Construction of overexpression vectors and heterologous expression of VdGRF5 resulted in larger leaf area,root elongation,and enhanced salt tolerance in Arabidopsis thaliana.Our study provides a reference for studying the role of VdGRFs in affecting blueberry growth and resistance to salt stress,and identifies a candidate GRF gene that regulates blueberry development and breeding for resistance to adversity.
作者
程芮
王彦文
王欣雨
周厚君
CHENG Rui;WANG Yanweng;WANG Xinyu;ZHOU Houjun(The Engineering Research Institute of Agriculture and Forestry,Ludong University,Yantai,Shandong 264025,China;College of Horticulture,Ludong University,Yantai,Shandong 264025,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2024年第11期1685-1696,共12页
Plant Physiology Journal
基金
国家自然科学基金青年基金项目(32001330)。
