摘要
为了验证HLAⅡ类基因是慢性HBV感染相关的遗传易感基因,深入了解不同人群慢性HBV感染的分子机制,从HLAⅡ类基因区域筛选出8个具有代表性的单核苷酸多态性(single nucleotide polymorphisms,SNP),重新分析所选SNP与慢性HBV感染的相关性。采用以医院为基础的病例对照研究,以183例慢性乙型肝炎(chronic hepatitis B,CHB)患者为病例组,196例HBV自限性感染者为对照组。采用SNaPshot技术对8个SNP进行等位基因分型。对照组与病例组比较,在显性模型下,rs9276370(GG+GT vs TT:P=0.013)、rs7756516(CC+CT vs TT:P=0.023)、rs7453920(AA+AG vs GG:P=0.003)、rs3077(AA+AG vs GG:P=0.028)、rs9277535(AA+AG vs GG:P=0.012)和rs9366816(TT+TC vs CC:P=0.014)多态性均与HBV自限性感染呈正相关,rs378352(AA+AG vs GG:P=0.024)则是CHB的危险因素。单倍型分析显示,HLA-DQ基因的rs9276370、rs7756516和rs7453920可以构成TTG和GCA 2种单倍型,TTG单倍型与CHB风险呈正相关,而GCA单倍型具有很强的HBV感染后清除作用。因此,该研究认为HLAⅡ基因多态性与慢性HBV感染存在相关性。
In order to verify the association between genetic susceptibility and chronic HBV infection with HLA classⅡgene and to understand the molecular mechanism of chronic HBV infection in different populations,this study screened 8 representative single nucleotide polymorphisms(SNPs)from the HLA classⅡgene region,and re-analyzed the correlation between the selected SNPs and the risk of chronic HBV infection.A hospital-based case-control study was carried out with 183 chronic hepatitis B(CHB)patients as the case group and 196 HBV self-limiting infection patients as the control group.Allele typing of selected SNPs was performed using SNaPshot technology.Dominant model analysis comparing the control group and case group revealed that rs9276370(GG+GT vs TT:P=0.013),rs7756516(CC+CT vs TT:P=0.023),rs7453920(AA+AG vs GG:P=0.003),rs3077(AA+AG vs GG:P=0.028),rs9277535(AA+AG vs GG:P=0.012),and rs9366816(TT+TC vs CC:P=0.014)polymorphisms were all positively correlated with HBV self-limiting infection.The rs378352(AA+AG vs GG:P=0.024)was a risk factor for CHB.Further haplotype analysis showed that rs9276370,rs7756516,and rs7453920 of HLA-DQ gene may exhibit haplotypes of TTG and GCA.Haplotype TTG was positively correlated with the risk of CHB,while haplotype GCA had a strong effect on clearance after HBV infection.Therefore,the results of this study suggest that HLAⅡpolymorphism may be associated with chronic HBV infection.
作者
孔凡辉
苏明宽
杨虹
吴水清
郭建峰
黄建成
KONG Fan-hui;SU Ming-kuan;YANG Hong;WU Shui-qing;GUO Jian-feng;HUANG Jian-cheng(Department of Laboratory Medicine,Mindong Hospital Affiliated to Fujian Medical University,Fuan 355000,China;Department of Gastroenterology,Mindong Hospital Affiliated to Fujian Medical University,Fuan 355000,China)
出处
《现代免疫学》
CAS
2024年第6期521-525,共5页
Current Immunology
基金
宁德市科技计划项目(20170013)。
