摘要
目的建立氯氰菊酯的8种对映异构体手性拆分方法。方法采用液相色谱-气相色谱-质谱联用法,通过Chiralcel OD-3[纤维素-三(3,5-二甲基苯基氨基甲酸酯)]手性柱,得到8种氯氰菊酯异构体的7个色谱峰,分为两组分析。采用液相色谱-气相色谱-质谱联用系统,将两个分离组分别通过进样针模式的LC-GC接口在线注入PTV进样口中实现浓缩,再使用气相色谱串联质谱方法进行异构体的完全分离。结果实现了氯氰菊酯8个对映异构体的完全分离。结论开发的液相色谱-气相色谱-质谱联用方法能够实现氯氰菊酯8种异构体的完全拆分,缩短了分析时间,提高了分离效率。
Objective This study aims to establish an effective chiral separation method for eight enantiomers of cypermethrin.Methods A liquid chromatography-gas chromatography-mass spectrometry(LC-GC-MS)approach was utilized to achieve separation,employing a Chiralcel OD-3 chiral column.Through this method,seven chromatographic peaks corresponding to chiral cypermethrin were obtained and subsequently categorized into two groups.The LC-GC-MS system was then employed to concentrate these two groups by utilizing an online injection into the PTV injection port via the LC-GC interface in injection needle mode.Complete separation of the isomers was achieved using GC-MS/MS.Results Complete separation of all eight enantiomers of cypermethrin was successfully achieved.Conclusions The developed LC-GC-MS method enables the complete separation of the eight isomers of cypermethrin,thereby reducing analysis time and enhancing separation efficiency.
作者
戚燕
张楠
赵旭东
刘平
赵榕
范赛
QI Yan;ZHANG Nan;ZHAO Xudong;LIU Ping;ZHAO Rong;FAN Sai(Beijing Center for Disease Prevention and Control,Beijing 100013,China;School of Public Health,Capital Medical University,Beijing 100069,China;不详)
出处
《首都公共卫生》
2024年第4期217-221,共5页
Capital Journal of Public Health
基金
国家自然科学基金青年基金项目(编号:81302431)。
关键词
氯氰菊酯
手性拆分
液相色谱
气相色谱串联质谱
Cypermethrin
Chiral separation
Liquid chromatography
Gas chromatography-mass spectrometry
