摘要
目的鉴定罗非鱼皮胶原蛋白抗氧化肽结构并阐明其机制。方法利用超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)鉴定罗非鱼皮胶原蛋白肽结构,通过计算机辅助虚拟筛选潜在抗氧化肽,以1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picryl-hydrazyl radical,DPPH)自由基、2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸[2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid),ABTS]阳离子自由基清除能力为指标进行体外抗氧化活性评价。结果罗非鱼皮胶原蛋白肽TMQTEADEE、EADIDGDGQ和SVDDAINMA具有抗氧化活性,其DPPH自由基清除能力半数抑制浓度(half-maximal inhibitory concentration,IC_(50))值分别为(2.71±0.07)、(4.79±0.24)和(3.88±0.10)mmol/L;其ABTS阳离子自由基清除能力IC_(50)值分别为(4.72±0.12)、(7.13±0.02)和(5.12±0.04)mmol/L。Keap1中的残基Arg380、Asn382、Arg415、Pro384、Ser383和Arg483是与罗非鱼皮胶原蛋白肽结合的关键氨基酸,其主要作用力是氢键和电荷相互作用。分子动力学模拟表明,肽可与Keap1蛋白紧密结合,具有稳定的结合构象。结论罗非鱼皮胶原蛋白肽TMQTEADEE、EADIDGDGQ和SVDDAINMA对DPPH自由基和ABTS阳离子自由基有抑制作用,具有一定的抗氧化活性。该研究为罗非鱼皮胶原蛋白肽抗氧化活性的开发利用提供了理论参考。
Objective To identify the antioxidant peptides from tilapia skin collagen and elucidate its action mechanism.Methods Tilapia skin collagen-derived peptides were characterized using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),and the peptides with potential antioxidant effect were screened by computer-assisted virtual screening,and their in vitro antioxidant activity was investigated using 1,1-diphenyl-2-picryl-hydrazyl radical(DPPH)and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid(ABTS))radical scavenging capacity.Results The results showed that the Tilapia skin collagen-derived peptides TMQTEADEE,EADIDGDGQ and SVDDAINMA possessed antioxidant activities,and their DPPH radical scavenging capacity IC_(50)values were(2.71±0.07),(4.79±0.24)and(3.88±0.10)mmol/L,respectively;and their ABTS radical scavenging capacity IC_(50)values were(4.72±0.12),(7.13±0.02)and(5.12±0.04)mmol/L.Residues Arg380,Asn382,Arg415,Pro384,Ser383 and Arg483 in Keap1 were the key amino acids binding to Tilapia skin collagen peptides,and the main forces involved was the interaction between hydrogen bond and attractive charges.Molecular dynamics simulations showed that the peptide bounds tightly to the Keap1 protein with a stable binding conformation.Conclusion The tilapia skin collagen peptides TMQTEADEE,EADIDGDGQ and SVDDAINMA show antioxidant activity by inhibiting DPPH radicals and ABTS cation radicals.This study provides a theoretical reference for the development and utilisation of antioxidant activity of tilapia skin collagen peptides.
作者
谭龙斌
张倩
于志鹏
郭恒文
赵文竹
TAN Long-Bin;ZHANG Qian;YU Zhi-Peng;GUO Heng-Wen;ZHAO Wen-Zhu(College of Food Science and Engineering,Hainan University,Haikou 570228,China;College of Food Science and Engineering,Bohai University,Jinzhou 121000;Hainan Pure Peptide Technology Co.,Ltd.,Haikou 570228,China)
出处
《食品安全质量检测学报》
CAS
2024年第18期190-198,共9页
Journal of Food Safety and Quality
基金
鱼胶原蛋白肽结构表征及活性筛选项目(RH2300003272)。
关键词
罗非鱼皮
胶原蛋白肽
抗氧化肽
虚拟筛选
分子对接
分子动力学模拟
Tilapia skin
collagen peptides
antioxidant peptide
virtual screening
molecular docking
molecular dynamics simulation
