摘要
[目的]为明确红叶杨品种全红杨与其亲本2025杨对叶锈病的抗性差异及其作用机制。[方法]通过在红叶杨品种全红杨和其亲本2025杨上人工接种北美栅锈菌,对两种杨树的发病状况、锈菌基因表达量、活性氧和抗氧化酶活性的动态变化进行了测定。[结果]表明两种杨树对北美栅锈菌均表现为感病,其中全红杨为中度感病,2025杨为高度感病。相比全红杨,锈菌在2025杨叶片内发育更快、潜育期更短、夏孢子堆更大更密。全红杨的3种抗氧化酶活性(POD、CAT、SOD)均高于2025杨,而2025杨中的活性氧水平高于全红杨。[结论]说明全红杨应对锈菌入侵较2025杨能更快的启动免疫应答反应,平衡活性氧水平,从而表现出较强的抗病性。
[Objective]To clarify the resistance and mechanism of red-leaved poplar P.deltoides cv.‘Quanhong’and its bud parental poplar P.deltoides‘Zhonglin 2025’against leaf rust,this study artificially inoculated Melampsora medusae Thüm on the Populus deltoides cv.‘Quanhong’and its parent P.deltoides‘Zhonglin 2025’.[Method]In this study,the rust resistance differences between P.deltoides cv.‘Quanhong’and P.deltoides‘Zhonglin 2025’,rust symptoms,gene expression of rust,dynamic changes of reactive oxygen species(ROS)and antioxidant enzyme activities in leaves of two poplars were determined.[Results]The results showed that both poplars behaved as susceptible cultivarietas against M.medusae,of which P.deltoides cv.‘Quanhong’was moderately susceptible and P.deltoides‘Zhonglin 2025’was highly susceptible.Compared with P.deltoides cv.‘Quanhong’,the rust clone developed faster in the leaves of P.deltoides‘Zhonglin 2025’,with shorter latent period,and larger and denser uredia.The activities of three antioxidant enzymes(POD,CAT,SOD)of P.deltoides cv.‘Quanhong’were higher than P.deltoides‘Zhonglin 2025’,while the ROS level was higher in P.deltoides‘Zhonglin 2025’.[Conclusion]The results suggests that P.deltoides cv.‘Quanhong’is able to activate innate immune response against M.medusae more quickly,balance ROS level and stronger disease resistance to rust accordingly.
作者
李春丽
彭子嘉
骆泽煜
余仲东
LI Chun-li;PENG Zi-jia;LUO Ze-yu;YU Zhong-dong(College of Forestry,Northwest A&F University,Yangling 712100,Shannxi,China)
出处
《林业科学研究》
CSCD
北大核心
2024年第5期178-185,共8页
Forest Research
基金
国家自然科学基金(No.31670650)。
关键词
红叶杨
北美栅锈菌
抗锈性
red-leaved poplar
Melampsora medusae
rust resistance
