摘要
建立高效液相色谱方法,使用C_(18)色谱柱,以甲醇-水(75∶25,V/V)为流动相,流速1.0 mL/min,糠醛和5-甲基糠醛的检测波长分别为271、284 nm,保留时间分别为4.969、9.246 min,可以同时准确测定糠醛和5-甲基糠醛的含量。结果表明:测定方法具有简便易操作、灵敏度高、重复性好的优点;优化了橡胶木中糠醛和5-甲基糠醛的提取方法,用高效液相色谱法测定了橡胶木素材和不同温度(125、155、185℃)热处理橡胶木中糠醛和5-甲基糠醛的含量变化。随着热处理温度升高,热处理橡胶木中糠醛和5-甲基糠醛的含量由3.4μg/g和1.9μg/g逐渐升高至103.5μg/g和54.1μg/g,此结果为调控热处理橡胶木工艺提供参考。
The established HPLC method used a C_(18)column with methanol-water(75:25,V/V)as the mobile phase at a flow rate of 1.0 mL/min.The detection wavelengths of furfural and 5-methylfurfural were 271 nm and 284nm,respectively,and their retention times were 4.969 min and 9.246 min.The furfural and 5-methylfurfural can be accurately deter mined at the same time by this method.The findings indicated that the method of determination offers the advantages of being simple and easy to operate,highly sensitive,and repeat-able;the extraction method of furfural and 5-methylfurfural in rubberwood was optimized,and the contents of fur-fural and 5-methylfurfural in thermally modified rubberwood at different temperatures(125℃,155℃,185℃)were deter mined.It's showed that the contents of furfural and 5-methylfurfural in thermally modified rubber-wood increased from 3.4μg/g and 1.9μg/g to 103.5μg/g and 54.1μg/g.This results would provide a theoretical reference for regulating the thermally modification technology of rubberwood.
作者
李彤彤
李冠君
李家宁
李晓文
李民
秦韶山
李雅超
Li Tongtong;Li Guanjun;Li Jianing;Li Xiaowen;Li Min;Qin Shaoshan;Li Yachao(Rubber Research Institute,Chinese Academy of Tropical Agricultural Science,Rubber Subcenter-National Center of Important Tropical Crops Engineering and Technology Research,Haikou Hainan 570100,China)
出处
《西南林业大学学报(自然科学)》
CAS
北大核心
2024年第5期215-220,共6页
Journal of Southwest Forestry University:Natural Sciences
基金
海南省自然科学基金项目(322QN412)资助。
关键词
橡胶木
热处理
糠醛
5-甲基糠醛
高效液相色谱法
rubberwood
thermally modification
furfural
5-methylfurfural
high performance liquid chro-matography
