摘要
目的探讨参附注射液辅助治疗对急性心肌梗死(AMI)大鼠NOD样受体蛋白3(NLRP3)/半胱氨酸天冬氨酸特异性蛋白酶1(Caspase-1)介导的细胞焦亡信号通路以及炎性因子水平的影响。方法40只大鼠随机分为假手术组、模型组、倍他乐克组(0.9 mg/kg)和联合组(倍他乐克0.9 mg/kg联合参附注射液6 mL/kg),每组10只,连续灌胃3周。检测造模前、造模后和治疗3周时大鼠血清肌钙蛋白I(cTnI)、肌酸激酶同工酶(CK-MB)、白细胞介素(IL)-6、IL-1β和肿瘤坏死因子-α(TNF-α)水平。造模后和治疗3周时,比较各组大鼠左心室射血分数(LVEF)、左心室收缩末期内径(LVESD)、左心室舒张末期内径(LVEDD)等心脏彩超参数以及心肌梗死面积。应用实时荧光定量聚合酶链式反应(qRT-PCR)及Western blot检测心肌梗死面积NLRP3、Caspase-1的mRNA及其蛋白表达水平。结果与假手术组相比,模型组造模后和治疗3周时cTnI、CK-MB、IL-6、IL-1β、TNF-α、心肌梗死面积、NLRP3 mRNA和Caspase-1 mRNA表达量均升高,差异有统计学意义(P<0.05);与模型组相比,倍他乐克组和联合组治疗3周时cTnI、CK-MB、IL-6、IL-1β、TNF-α、心肌梗死面积、NLRP3 mRNA和Caspase-1 mRNA表达量均降低,且联合组上述指标均低于倍他乐克组,差异有统计学意义(P<0.05)。结论参附注射液辅助治疗AMI能够进一步减轻心肌细胞损伤,缩小梗死面积,抑制炎症反应和细胞焦亡活性。
Objective To investigate the effect of adjuvant therapy of Shenfu Injection on NOD-like receptor protein 3(NLRP3)/cysteine-aspartic acid-specific protease 1(Caspase-1)mediated pyroptosis signaling pathway and inflammatory factor levels in rats with acute myocardial infarction(AMI).Methods A total of 40 rats were randomly divided into sham operation group,model group,betaloc group(0.9 mg/kg),and combination group(0.9 mg/kg betaloc combined with 6 mL/kg Shenfu Injection),with 10 rats in each group.The rats were treated by gavage continuously for 3 weeks.The levels of serum troponin I(cTnI),creatine kinase-MB(CK-MB),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)in rats were detected before modeling,after modeling,and at 3 weeks of treatment.Echocardiographic parameters such as left ventricular ejection fraction(LVEF),left ventricular end-systolic diameter(LVESD),left ventricular end-diastolic diameter(LVEDD),and myocardial infarction area were compared among groups after modeling and at 3 weeks of treatment.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)and Western blot were used to detect the mRNA and protein expression levels of NLRP3 and Caspase-1 in the myocardium.Results Compared with the sham operation group,the levels of cTnI,CK-MB,IL-6,IL-1β,TNF-α,myocardial infarction area,and the expressions of NLRP3 mRNA and Caspase-1 mRNA in the model group were significantly increased after modeling and at 3 weeks of treatment(P<0.05);compared with the model group,the levels of cTnI,CK-MB,IL-6,IL-1β,TNF-α,myocardial infarction area,and the expressions of NLRP3 mRNA and Caspase-1 mRNA in the betaloc group and the combination group were significantly decreased at 3 weeks of treatment,and the above indicators in the combination group were significantly lower than those in the betaloc group(P<0.05).Conclusion Adjuvant therapy of Shenfu Injection for AMI can further alleviate myocardial cell injury,shorten infarction size,and inhibit inflammatory response and pyroptosis activity.
作者
兰友玲
李天发
占亚童
陈燕娥
关富清
杨洋
LAN Youling;LI Tianfa;ZHAN Yatong;CHEN Yan′e;GUAN Fuqing;YANG Yang(the First Department of Cardiology,the First Affiliated Hospital of Hainan Medical University,Haikou,Hainan,570102;Department of Respiratory Medicine,the First Affiliated Hospital of Hainan Medical University,Haikou,Hainan,570102)
出处
《实用临床医药杂志》
CAS
2024年第15期3-8,13,共7页
Journal of Clinical Medicine in Practice
基金
国家自然科学地区科学基金项目(81860075)
海南医学院第一附属医院青年培育基金项目(HYYFYPY202305)。
