摘要
目的探讨热休克蛋白90α(heat shock protein 90α,Hsp90α)在结肠癌中的表达及潜在的临床价值。方法采用生物信息学和免疫组化法分析结肠癌中Hsp90α的表达水平,及其与临床病理学特征、预后和免疫细胞浸润水平的关系;采用CCK-8细胞增殖实验和平板克隆实验检测敲除Hsp90AA1前后结肠癌细胞的增殖能力。结果生物信息学分析结果显示,Hsp90AA1在结肠癌组织中异常高表达,其表达水平越高,患者预后越差;Hsp90AA1表达与CD4^(+)T细胞(Th2)、CD8^(+)T细胞、髓样抑制细胞、Tregs细胞、中性粒细胞、巨噬细胞、M1巨噬细胞、M2巨噬细胞的浸润水平呈正相关;免疫组化结果显示结肠癌组织中Hsp90α表达明显高于癌旁正常组织,Hsp90α表达与患者性别、肿瘤大小、位置、分化程度、TNM分期、淋巴结转移、脉管癌栓、神经侵犯、远处转移等无关(P>0.05),与结肠癌患者年龄具有相关性(P<0.05)。Hsp90α高表达是影响结肠癌患者预后的独立危险因素。细胞实验结果显示,敲除Hsp90AA1可抑制结肠癌细胞的生长及增殖能力。结论Hsp90α在结肠癌中高表达,可能是结肠癌预后不良的潜在分子学标志物。
Purpose To investigate the expression and potential clinical value of heat shock protein 90α(Hsp90α)in colon adenocarcinoma.Methods The expression level of Hsp90αin colon adenocarcinoma and its relationship with clinicopathological features,prognosis and immune cell infiltration were analyzed by bioinformatics and immunohistochemistry.The proliferation ability of colon cancer cells before and after Hsp90AA1 knockout was detected by CCK-8 cell proliferation assay and plate cloning assay.Results The bioinformatics tools showed that Hsp90AA1 was abnormally higher in colon cancer tissues than adjacent tissues,and the higher the expression level,the worse the prognosis of patients.The expression of Hsp90AA1 was positively correlated with the infiltration levels of CD4^(+)T cells(Th2),CD8^(+)T cells,myeloid inhibitory cells,Tregs cells,neutrophils,macrophages,M1 macrophages and M2 macrophages.Immunohistochemical results showed that the expression of[CD44]Hsp90αin colon cancer tissues was significantly higher than in its adjacent tissues.The expression of Hsp90αwas related to age(P<0.05),not related to gender,tumor size,location,clinical classification,differentiation degree,tumor node metastasis,lympho-vascular invasion,perineural invasion(P>0.05).The high expression of Hsp90αwas an independent risk factor for the prognosis of colon cancer patients.The results of cell experiments showed that Hsp90AA1 knockout inhibited the growth and proliferation of colon cancer cells.Conclusion Hsp90αis highly expressed in colon adenocarcinoma,which may be a potential molecular marker for poor prognosis of colon adenocarcinoma.
作者
张阳
吴晓婷
王芳
戴星辰
张玉环
高玉婧
ZHANG Yang;WU Xiaoting;WANG Fang;DAI Xingchen;ZHANG Yuhuan;GAO Yujing(the First Clinical Medical College,Ningxia Medical University,Yinchuan 750004,China;Department of Gastroenterology,General Hospital of Ningxia Medical University,Yinchuan 750004,China;Department of Digestive Endoscopy,Xi’an International Medical Center Hospital,Xi’an 710100,China;Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Ningxia Medical University,Yinchuan 750004,China)
出处
《临床与实验病理学杂志》
CAS
北大核心
2024年第8期845-852,共8页
Chinese Journal of Clinical and Experimental Pathology
基金
国家自然科学基金(81860442)。
