摘要
【目的】分析国内外引种石斛种质资源遗传多样性,揭示不同种质间的亲缘关系,为促进石斛种质的有效利用及保护提供参考。【方法】利用ISSR分子标记技术对杯鞘石斛、金钗石斛、檀香石斛等50种石斛进行遗传多样性和亲缘关系研究分析,并构建DNA指纹图谱。【结果】9条多态性高、重复性好的引物共扩增出112个等位基因,其中,多态性位点102个,多态性比率为93.09%。检测的位点数为9~14,平均为12.44;50种石斛属植物的遗传相似系数为0.15~0.95。聚类分析显示,在遗传相关系数0.70处,可将石斛材料分为12个大组。PCR扩增显示,引物UBC808可将50种石斛完全区分开,基于其扩增的14个清晰的多态性条带构建50种石斛的DNA指纹图谱。【结论】50种石斛种间具有丰富的遗传多样性。
【Objective】The genetic diversity of Dendrobium germplasm resources introduced domestically and internationally was analyzed to uncover the genetic relationship among different germplasms,and to provide a reference for promoting effective utilization and preservation of Dendrobium germplasm.【Method】The genetic diversity and genetic relationship among 50 Dendrobium species,including Dendrobium gratiosissimum,D.nobile,D.anosmum,etc.,were analyzed by ISSR molecular marker technology,as well as a DNA fingerprint was constructed.【Result】9 primers with high polymorphism and good repeatability amplified a total of 112 alleles,including 102 polymorphism sites and a polymorphism ratio of 93.09%.The number of detected alleles ranged from 9 to 14,with an average of 12.44.The genetic similarity coefficients among the 50 Dendrobium species ranged from 0.15 to 0.95.The cluster analysis revealed that the 50 Dendrobium species could be divided into 12 groups at a genetic similarity coefficient of 0.70.PCR amplification showed that primer UBC808 successfully distinguished all 50 Dendrobium species.Additionally,DNA fingerprint of the 50 Dendrobium species was constructed based on 14 clear polymorphic bands amplified with this primer.【Conclusion】The 50 Dendrobium species have abundant genetic diversity.
作者
江荣慧
田凡
王莲辉
罗在柒
颜凤霞
JIANG Ronghui;TIAN Fan;WANG Lianhui;LUO Zaiqi;YAN Fengxia(Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Karst Mountainous Areas of Southwestern China,Guizhou Academy of Forestry,Guiyang,Guizhou 550005,China)
出处
《贵州农业科学》
CAS
2024年第7期63-70,共8页
Guizhou Agricultural Sciences
基金
贵州省林业科研课题项目“石斛属植物资源调查及引种保存技术研究”(黔林科合[2019]01号)
“不同栽培模式金钗石斛石斛碱含量研究”(黔林科合J字[2022]11号)
“贵州石斛产业发展近野生栽培试验基地建设项目
贵州石斛产业发展近野生栽培试验基地建设(新增试验点)”(黔石科合2019001)
“贵州石斛种质资源库建设项目”(黔石科合2019008)
“不同附生树种对铁皮石斛生长及代谢成分响应技术研究”(黔林科合[2019]02号)
“几种石斛种质资源收集保存及繁育技术研究”(黔林科合[2021]02号)。
