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分子生物学技术在病原微生物检验中的应用研究

Research on the application of molecular biology technology in the detection of pathogenic microorganisms
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摘要 目的探析分子生物学技术在病原微生物检验中的应用效果。方法将2022年7月至2023年6月间在该公司进行病原微生物检验的患者作为试验对象,计入100例,均予以实施荧光RT-PCR法检验、常规反转录RT-PCR法检验,分别计入试验组、对照组,对比两组检验效果。结果试验组阳性检出率明显高于对照组,差异有统计学意义(P<0.05)。结论在病原微生物检验中,分子生物学技术的应用可有效提升阳性检出率,特别是分子生物学技术的合理选用,可为临床后续治疗方案的设定提供更充分、可靠的参考依据。 Objective To explore the application effect of molecular biology technology in the detection of pathogenic microorganisms.Methods Patients who underwent pathogenic microorganism testing in our company from July 2022 to June 2023 were selected as the experimental subjects,and 100 cases were included in the study.Fluorescence RT-PCR and conventional reverse transcription RT-PCR were performed,and the results were compared between the experimental group and the control group.Results The positive detection rate of the experimental group was significantly higher than that of the control group,and there was a significant difference between the groups(P<0.05).Conclusion In the detection of pathogenic microorganisms,the application of molecular biology technology can effectively improve the positive detection rate,especially the reasonable selection of molecular biology technology,which can provide more comprehensive and reliable reference basis for the setting of clinical follow-up treatment plans.
作者 王新宇 WANG Xinyu(Chengdu Boao Independent Medical Laboratory Co.,Ltd.,Chengdu,Sichuan 611130,China)
出处 《国际检验医学杂志》 CAS 2024年第S01期146-148,共3页 International Journal of Laboratory Medicine
关键词 分子生物学技术 病原微生物 微生物检验 检验价值 molecular biology technology pathogenic microorganisms microbial testing test value
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