摘要
目的 探讨微小RNA-26(miR-26)、转化生长因子β1(TGF-β1)、蛋白酶体激活因子11S调节蛋白复合物γ亚单位(REGγ)在子宫内膜癌组织中的表达及相关性。方法 收集60例子宫内膜癌组织(内膜癌组)、20例子宫内膜增生组织(内膜增生组)、20例正常增殖期子宫内膜组织(正常组),实时荧光定量PCR(qPCR)法检测子宫内膜组织中miR-26、TGF-β1、REGγ表达水平。比较三组子宫内膜组织中miR-26、TGF-β1、REGγ表达水平差异;收集子宫内膜癌患者临床病理特征,分析其临床病理参数与miR-26、TGF-β1、REGγ表达水平关系;Pearson法分析子宫内膜癌组织miR-26、TGF-β1、REGγ表达水平之间及其与临床分期、分化程度的相关性;绘制受试者工作特征(ROC)曲线分析miR-26、TGF-β1、REGγ表达水平对子宫内膜癌的诊断价值。结果 正常组、内膜增生组、内膜癌组,miR-26表达水平依次降低,TGF-β1、REGγ表达水平依次升高,组间比较差异有统计学意义(P<0.001)。子宫内膜癌组织中的miR-26、TGF-β1、REGγ表达水平与其临床分期、组织分化和淋巴结转移有关(P<0.05),与肌层浸润程度无关(P>0.05)。子宫内膜癌组织miR-26表达水平与TGF-β1、REGγ表达水平均呈负相关关系(P<0.001),而TGF-β1、REGγ表达水平之间呈正相关关系(P<0.001);miR-26表达水平与临床分期呈负相关关系,与分化程度呈正相关关系(P<0.001);TGF-β1、REGγ表达水平与临床分期呈正相关关系,与分化程度呈负相关关系(P<0.001)。子宫内膜癌患者miR-26、TGF-β1、REGγ表达水平诊断子宫内膜癌的AUC分别是0.705/0.745/0.760,低于联合诊断方法的0.861。结论 miR-26、TGF-β1、REGγ在子宫内膜癌组织中表达异常,与临床分期、组织分化程度相关,且三者之间互相相关,联合用于诊断子宫内膜癌的临床价值显著。
Objective To investigate the expression and correlation of microRNA-26(miR-26),Transforming growth factorβ1(TGF-β1)and proteasome activator 11S regulator complex gamma subunit(REGγ)in endometrial cancer.Methods 60 cases of endometrial carcinoma(endometrial carcinoma group),20 cases of endometrial hyperplasia(endometrial hyperplasia group)and 20 cases of normal proliferative endometrium(normal group)were collected.Real-time quantitative PCR(RT-qPCR)was used to detect the expression of miR-26,TGF-β1 and REGγin endometrial tissue.The expression levels of miR-26,TGFΒ1 and REGγwere compared among the 3 groups,and the clinicopathologic features of the endometrial cancer were collected to analyze the relationship between the clinicopathological parameters and the expression levels of miR-26,TGFΒ1 and REGγ.The expression levels of miR-26,TGF-β1 and REGγin endometrial cancer tissues were analyzed by Pearson's method The diagnostic value of miR-26,TGF-β1 and REGγin endometrial cancer was analyzed by receiver operating characteristics curve(ROC).Results The expression levels of miR-26 in normal group,endometrial hyperplasia group and endometrial carcinoma group decreased in turn,while the expression levels of TGF-β1 and REGγincreased in turn,there was significant difference among the 3groups(P<0.001).The expression of miR-26,TGF-β1 and REGγin the pathological tissues of endometrial cancer patients was correlated with clinical stage,histological differentiation and lymph node metastasis(P<0.05),but not with the degree of myometrial invasion(P>0.05).The expression of miR-26 was negatively correlated with the expression of TGF-β1 and REGγin endometrial cancer patients(P<0.001),while the expression of TGF-β1 and REGγwas positively correlated(P<0.001).The expression level of miR-26 was negatively correlated with clinical stage and positively correlated with differentiation(P<0.001),[LM]and the expression level of TGFΒ1 and REGγwas positively correlated with clinical stage and negatively correlated with differentiation(P<0.001).The AUC of miR-26,TGF-β1 and REGγwere 0.705,0.7450,0.760,respectively,which was lower than 0.861 of the combined diagnostic endometrial cancer in endometrial cancer patients.Conclusion The abnormal expression of miR-26,TGF-β1 and REGγin endometrial carcinoma tissue is related to clinical stage and degree of tissue differentiation,and they are interrelated,so the combination of them has significant clinical value in diagnosing endometrial carcinoma.
作者
许佳佳
陈英华
王丽元
XU Jiajia;CHEN Yinghua;WANG Liyuan(General Hospital of Pingmei Shenma Medical Group,Pingdingshan,467000)
出处
《实用癌症杂志》
2024年第7期1105-1109,共5页
The Practical Journal of Cancer
