摘要
以青海省6种小檗属植物的休眠枝条为试材,采用人工梯度降温的方法,分别在-20℃、-25℃、-30℃、-35℃、-40℃下处理24 h,以4℃为对照。研究了低温处理后各小檗枝条各项生理指标。结果表明:随着处理温度的降低,拟小檗的相对电导率呈持续下降趋势,鲜黄小檗的相对电导率呈持续上升趋势,刺檗、直穗小檗、匙叶小檗、细叶小檗的相对电导率呈先上升后下降趋势;拟小檗、匙叶小檗PRO含量呈持续上升趋势,刺檗、鲜黄小檗、直穗小檗、细叶小檗PRO含量呈持续下降趋势;MDA、POD、SOD、SS、SP变化一致,呈先上升后下降趋势。通过计算各单项指标的抗寒系数,建立隶属函数进行评价,评价结果为鲜黄小檗﹥直穗小檗﹥刺檗﹥拟小檗﹥匙叶小檗﹥细叶小檗。
The dormant branches of six Berberis plants in Qinghai Province were used as test materials,and the artificial gradient cooling method was used to treat them at-20°C,-25°C,-30°C,-35°C,-40°C for 24 h,with 4℃ as the control.The physiological indexes of Berberis branches after low temperature treatment were studied.The results showed that with the decrease of treatment temperature,the relative conductivity of Berberis dubia showed a continuous downward trend,the relative conductivity of Berberis diaphana showed a continuous upward trend,and the relative conductivity of Berberis vulgaris,Berberis dasystachya,Berberis vernae and Berberis poiretii showed a trend of increasing first and then decreasing.The PRO content of Berberis dubia and Berberis vernae showed a continuous upward trend,while the PRO content of Berberis vulgaris,Berberis diaphana,Berberis dasystachya and Berberis poiretii showed a continuous downward trend.The changes of MDA,POD,SOD,SS and SP were consistent,showing a trend of increasing first and then decreasing.By calculating the cold resistance coefficient of each single index,the membership function is established for evaluation.The evaluation results were as follows:Berberis diaphana>Berberis dasystachya>Berberis vulgaris>Berberis dubia>Berberis vernae>Berberis poiretii.
作者
田新格
何振华
顾文毅
祁银燕
Tian Xinge;He Zhenhua;Gu Wenyi;Qi Yinyan(Academy of Agriculture and Forestry Sciences,Qinghai University/Qinghai Academy of Agriculture and Forestry Sciences,Tree Genetics and Breeding Laboratory of Qinghai Plateau,Key Laboratory of Tree Genetics and Breeding of Qinghai Plateau,National Forestry and Grassland Administration,Xining 810016,China;Xunhua Salar Autonomous County Natural Resources and Forestry Grassland Bureau,Haidong 811100,China;Research and Utilization Laboratory of Qinghai-Tibet Plateau Germplasm Resources,Xining 810016,China)
出处
《青海科技》
2024年第2期161-168,共8页
Qinghai Science and Technology
关键词
小檗
人工低温胁迫
隶属函数法
Berberis
Artificial low temperature stress
Membership function method
