摘要
目的基于特征离子分析川陈皮素在大鼠体内代谢产物。方法10只大鼠随机分为给药组和空白组,分别灌胃给予川陈皮素0.5%CMC-Na混悬液(250 mg/kg)和0.5%CMC-Na溶液,采集血浆、尿液和粪便,固相萃取法前处理,进行UHPLC-HRMS分析。根据诊断产物离子、色谱保留时间、精确分子量和中性丢失碎片对代谢物进行系统描述,以-CH_(3)(m/z 15)特征离子为诱饵,在代谢物数据集中获得准确的代谢物。结果共鉴定出64个代谢物,主要代谢途径为葡萄糖醛酸化、硫酸化、氢化及其复合反应。结论本实验阐明了川陈皮素在大鼠体内代谢产物,为其进一步开发提供了新的借鉴。
AIM To analyze the metabolites of nobiletin in rats in vivo based on characteristic ions.METHODS Ten rats were assigned into administration group and control group,and given intragastric administration of the 0.5%CMC-Na suspension of nobiletin(250 mg/kg)and 0.5%CMC-Na solution,respectively,after which plasma,urine and feces were collected,solid phase extraction method was adopted in pretreatment,UHPLC-HRMS analysis was performed.The candidate metabolites were systematically described according to diagnostic product ions,chromatographic retention time,accurate molecular weight and neutral loss fragments,after which accurate metabolites were obtained in the established metabolite data set with-CH_(3)(m/z 15)characteristic ions as a baits.RESULTS A total of 64 metabolites were identified,whose main metabolic pathways were glucuronidation,sulfation,hydrogenation and their compound reactions.CONCLUSION This experiment elucidates the metabolites of nobiletin in rats in vivo,which provides a new reference for its further development.
作者
李喆
王雨晴
吴冬雪
李双凤
李亚男
王少平
张加余
代龙
LI Zhe;WANG Yu-qing;WU Dong-xue;LI Shuang-feng;LI Ya-nan;WANG Shao-ping;ZHANG Jia-yu;DAI Long(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250300,China;College of Pharmacy,Binzhou Medical University,Yantai 264003,China)
出处
《中成药》
CAS
CSCD
北大核心
2024年第6期1800-1809,共10页
Chinese Traditional Patent Medicine
基金
国家自然科学基金青年科学基金项目(82304815)
山东省泰山学者青年专家项目(tsqn202103110)
山东省青创人才引育计划——中药复杂体系作用模式解析创新研究团队项目(10073004)。
