摘要
为明确菌株QZ-1对番茄灰霉病的生防潜力,以山东青州桃树根际土壤中分离的拮抗细菌QZ-1为研究对象,通过平板试验、果实接种试验,研究该菌株对多种作物病原真菌的拮抗以及对番茄灰霉病的防治作用。结果表明:QZ-1抑菌谱广,对9种植物病原真菌均有不同程度的抑制作用,该菌株无菌滤液可显著抑制番茄灰霉病菌、桃枝枯病菌和油菜菌核病菌的菌丝生长,抑菌率分别为80.00%、77.33%和76.67%;且在番茄果实上对灰霉病有较好的防病效果。通过比对拮抗细菌QZ-116S rDNA和rpoB序列,发现该细菌与贝莱斯芽胞杆菌(Bacillus velezensis)和解淀粉芽胞杆菌(B.amyloliquefaciens)相似度最高,均达到99%。结合生理生化检测结果,初步鉴定菌株QZ-1为贝莱斯芽胞杆菌。PCR扩增结果显示,QZ-1基因组DNA中可扩增到1203 bp伊枯草菌素A的ituD基因和1400 bp丰原素的fenB基因。质谱分析表明,细菌发酵液中抗菌物质主要成分为伊枯草菌素A及其同系物。综上,贝莱斯芽胞杆菌QZ-1株主要通过产生伊枯草菌素而有效抑制番茄灰霉病菌的扩展,为番茄灰霉病的有效防控提供新的生防资源。
In order to clarify the biocontrol potential of strain QZ-1 against tomato gray mold,the antagonistic bacterium QZ-1,isolated from the rhizosphere soil of peach trees in Qingzhou,Shandong Province,the antifungal activity was determined.The antagonistic effects of strain QZ-1 on various phytopathogenic fungi and the control effect against tomato gray mold were assayed using plate confrontation assays and fruit inoculation tests.The results showed that strain QZ-1 displayed different degrees of inhibitary ability against tested plant pathogenic fungi.Moreover,the sterile filtrate of QZ-1 could significantly inhibit mycelial growth of B.cinerea,Phomopsis amygdali and Sclerotinia sclerotiorum,and the inhibition rates were 80.00%,77.33%and 76.67%respectively.QZ-1 also exhibited a good biological control effect to B.cinerea in tomato fruit.Strain QZ-1 displayed 99%similarities with that of Bacillus velezensis and B.amyloliquefaciens based on the 16S rDNA and rpoB sequence analysis.Combined with the physiological and biochemical traits,the strain QZ-1 was identified as B.velezensis.PCR amplification results showed that the presence of 1203 bp ituD and 1400 bp fenB genes,coding for iturin A and fengycin,in the genomic DNA of QZ-1.Mass spectrometry analysis suggested that the iturin A and its homologues were the main antimicrobial compounds produced by QZ-1.In summary,B.velezensis QZ-1 effectively inhibits the mycelia development of B.cinerea through the iturin A production,and provides an excellent new biocontrol resource for controlling of tomato gray mold.
作者
宋晓雅
毛子玥
温德宇
朱宇昕
张清霞
SONG Xiaoya;MAO Ziyue;WEN Deyu;ZHU Yuxin;ZHANG Qingxia(College of Plant Protection,Yangzhou University,Yangzhou 225509,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2024年第2期93-101,共9页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(32072471)
扬州市科技计划(现代农业)项目(YZ2023056)。
