摘要
以3种商品茶多酚(CTP)为研究对象,对其儿茶素类化合物组分及其结构进行表征,并探究其抗氧化性和抑菌效果。液相质谱测定结果表明,3种CTP均检出7种儿茶素类化合物,其中表没食子儿茶素没食子酸酯(EGCG)含量最高,CTP1-CTP3含量分别为40.12%、36.27%和59.13%。SEM图谱分析发现,3种CTP均呈现出大小不一和棱角分明的块状结构。红外光谱分析显示,3种CTP分别在1342~1312和1141 cm^(-1)处出现酚羟基的特征峰,且以CTP3峰值强度最高,依次高于CTP1和CTP2。CTP3对DPPH、·OH、ABTS自由基的半抑制浓度最低,分别为0.0876、0.0605、0.1525 mg·mL^(-1)。CTP3对金黄色葡萄球菌和沙门菌的抑菌效果最佳,其抑菌圈直径分别为23.24和11.43 mm。这一研究为CTP作为抗氧化剂和抑菌剂在食品加工中的开发和应用提供了参考。
Three commercial tea polyphenols(CTP)were used as research objects to characterize their catechin-based compound components and characterizations,and their antioxidant activities and antibacterial effects were further explored.Liquid phase mass spectrometry's result indicated that,7 kinds of catechin-based compounds were detected in all three CTP,with the highest relative content of epigallocatechin gallate(EGCG)component being 40.12%,36.27%and 59.13%for CTP1-CTP3,respectively.SEM test found that,all three CTP exhibited block structures with different sizes and sharp edges.Infrareds pectrum analysis showed that,the characteristic peaks of phenolic hydroxyl group occurred at the respective 1342-1312 and 1141 cm^(-1)for all three CTP,and their peak intensity sequence followed CTP3>CTP1>CTP2.Half inhibition concentration of CTP3 to DPPH,·OH and ABTS free radical were lowest with 0.0876,0.0605 and 0.1525 mg·mL^(-1),respectively.CTP3 possessed the best antibacterial effects on the Staphylococcus aureus and Salmonella with 23.24 and 11.43 mm of inhibitory zone diameter,respectively.This research results could provide a theoretical reference for CTP as antioxidants and antibacterial agents in the development and application of food processing.
作者
刘雅楠
张迅
单洪雁
赵文鹏
李松南
王君
LIU Yanan;ZHANG Xun;SHAN Hongyan;ZHAO Wenpeng;LI Songnan;WANG Jun(College of Tourism and Cuisine,Yangzhou University,Yangzhou 225127,China;Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China,Institutes of Agricultural Science and Technology Development,Yangzhou University,Yangzhou 225009,China)
出处
《扬州大学学报（农业与生命科学版）》
CAS
北大核心
2024年第2期84-92,共9页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(82000791)
江苏省自然科学基金资助项目(BK20220585)
中国博士后自然科学基金面上项目(2022M712692)
扬州市重点研发计划(社会发展)项目(YZ2022076)
扬州市“绿扬金凤计划”项目(YZLYJFJH2021YXBS172)
扬州大学大学生科技创新基金项目(X20220894)。
关键词
茶多酚
液相质谱
红外光谱
自由基清除
抑菌圈
tea polyphenols
liquid phase mass spectrometry
infrared spectrum
free radical scavenging
inhibition zone
