摘要
采用改良的QuEChERS方法进行前处理,建立了甘薯和土壤中烯效唑残留的QuEChERS-高效液相色谱-串联质谱检测方法。样品用含0.1%乙酸的乙腈振荡提取,经C18净化剂进行基质净化,采用C18反相色谱柱分离,进行LC-MS/MS检测。结果表明:烯效唑在0.0005~1.0 mg/L浓度范围内,质量浓度与对应的峰面积间呈良好的线性关系,相关系数为0.9951~0.9959;在0.002、0.02、0.20 mg/kg 3个添加水平下,烯效唑在甘薯和土壤中的平均回收率为90.3%~106.7%,相对标准偏差为0.6%~3.6%,方法定量限为0.002 mg/kg。该方法方便快捷、精准定量、检测灵敏,适用于甘薯和土壤样品中烯效唑的检测。
A method for determining the uniconazole residue in sweet potatoes and soil was established by the combination of improved QuEChERS(quick,easy,cheap,effective,rugged,and safe)with high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Each sample was extracted by shaking with acetonitrile containing 0.1%(volume fraction)acetic acid,purified by C18 purification agent,separated by reversed-phase C18 column chromatography,and detected by LC-MS/MS.The results showed good linearity between the peak area and the uniconazole concentration within the range of 0.0005-1.0 mg/L(R2 of 0.9951-0.9959).The recovery of uniconazole in sweet potatoes and soil at the spike levels of 0.002,0.02,and 0.20 mg/kg ranged from 90.3%to 106.7%with the relative standard deviations(RSDs)of 0.6%-3.6%.The established method showed the limit of quantification(LOQ)of 0.002 mg/kg.With easy operation,high sensitivity,and high accuracy,the established method is suitable for the detection of uniconazole in sweet potatoes and soil.
作者
左仁芳
戴桂金
王妙
张玉涵
罗杰
熊浩
刘志邦
陈武瑛
张卓
ZUO Ren-fang;DAI Gui-jin;WANG Miao;ZHANG Yu-han;LUO Jie;XIONG Hao;LIU Zhi-bang;CHEN Wu-ying;ZHANG Zhuo(Plant Protection and Inspection Station,Fenghuang County Bureau of Agriculture and Rural Affairs,Fenghuang 416200,PRC;Agricultural Characteristic Industry Service Center,Fenghuang County Bureau of Agriculture and Rural Affairs,Fenghuang 416299,PRC;Hunan Institute of Plant Protection,Hunan Academy of Agricultural Sciences,Changsha 410125,PRC)
出处
《湖南农业科学》
2024年第5期90-95,共6页
Hunan Agricultural Sciences
基金
国家自然科学基金项目(32202340)。
关键词
烯效唑
甘薯
高效液相色谱-串联质谱
残留
方法
uniconazole
sweet potato
high-performance liquid chromatography-tandem mass spectrometry
residue
method
