摘要
目的探讨维生素C(vitamin C,VC)对高温暴露引起大鼠免疫功能失调的干预效果。方法24只SPF级的8 w龄雄性SD大鼠,适应性喂养1 w后,随机分为常温对照组(NC)、高温暴露组(HE)、HE+VC 10 mg/kg组和HE+VC 14 mg/kg组,每组6只。两个干预组每天早晨按1.0 ml/100 g体质量给予1.0、1.4 g/L的VC溶液灌胃,其它组给予等量蒸馏水灌胃;各HE组每日上、下午进行湿球黑球温度(wet bulb globe temperature,WBGT)指数(22±1)℃、(33±1)℃环境下的跑台运动,每次30 min,每周6 d;连续4 w。于实验第29 d处死各组大鼠,采用流式细胞术检测外周血淋巴细胞亚群CD3^(+)、CD4^(+)、CD8^(+)和CD16^(+)和CD45^(+)占比,采用酶联免疫法测定血清中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素(interleukin,IL)-2和IL-6水平、以及免疫球蛋白(immunoglobulin,Ig)A、IgG、IgM和补体3(complement 3,C3)浓度。结果HE组全血中T细胞百分比及CD4^(+)/CD8^(+)比值、NK细胞和WBC百分比、血清中IL-6、IgA、IgG和C3浓度均低于NC组(均P<0.05),HE组IL-2浓度显著高于NC组(P<0.05)。HE+VC 10mg/kg组、HE+VC 14 mg/kg组T细胞和NK细胞百分比均显著高于HE组(均P<0.05),HE+VC 14 mg/kg组CD4^(+)/CD8^(+)、WBC百分比、TNF-α、IL-6、IgA、IgG、IgM和C3浓度均显著高于HE组(均P<0.05);两个给予VC组IL-2浓度与HE组比较均无显著变化(P>0.05)。结论高温暴露对大鼠的细胞和体液免疫功能产生影响,同时影响相应免疫因子释放水平;14 mg/kg VC干预可减轻高温引起的大鼠免疫水平失调。
Objective To investigate the effects of vitamin C(VC)intervention on immune function in rats exposed to high-temperature.Methods Twenty-four SPF 8-week-old Sprague-Dawley(SD)rats were randomly divided into four groups:normal temperature control(NC),high temperature exposure(HE),HE supplemented with 10 mg/kg VC and 14 mg/kg VC.Two intervention groups were administrated intragastrically with 1.0 ml VC solution at the concentration of 1.0 g/L or1.4 g/L,and the other groups were given the same amount of distilled water every morning.The rats ran on a treadmill in a normal environment(22±1)℃or a heat environment(33±1)℃for 30 min in the morning and in the afternoon daily,consecutive six days once a week for four weeks.The rats were sacrificed on the 29th day.Flow cytometry was used to analyze the proportions of CD3^(+),CD4^(+),CD8^(+),CD16^(+)and CD45^(+)cells in peripheral blood.The levels of IgA,IgG,IgM,C3,and TNF-α,IL-2,IL-6 in the serum were measured by enzyme-linked immuosorbent assay.Results Compared with the NC group,the percentages of T cells,NK cells,WBC,CD4^(+)/CD8^(+)ratio in the blood,and the contents of IL-6,IgA,IgG and C3 in the serum were significantly decreased.The IL-2 level was significantly increased in the HE group(all P<0.05).Compared with the HE group,the percentages of T cells and NK cells in the 10 mg/kg VC and 14 mg/kg VC groups,as well as CD4^(+)/CD8^(+)ratio,the percentage of WBC,the contents of IgA,IgG,IgM,C3,TNF-αand IL-6 in the 14 mg/kg VC group were significantly increased(P<0.05).There was no significant difference in the content of IL-2 between two VC supplementation groups and the HE group.Conclusion High temperature exposure has profound effect on both cell-mediated immunity and humoral immunity in rats.The intervention by 14 mg/kg VC can alleviate those negative effects caused by high temperature exposure.
作者
杜葳
江小华
田景瑞
李云
范红敏
DU Wei;JIANG Xiao-hua;TIAN Jing-rui;LI Yun;FAN Hong-min(School of Public Health,North China University of Science and Technology,Tangshan 063210;School of Basic Medical Sciences,North China University of Science and Technology,Tangshan 063210,China)
出处
《营养学报》
CAS
CSCD
北大核心
2024年第1期28-33,共6页
Acta Nutrimenta Sinica
基金
河北省重点研发计划项目(No.192777129D)
唐山市科技创新团队培养计划(No.20130206D)。
关键词
维生素C
免疫功能
高温
大鼠
vitamin C
immunefunction
high temperature
rat
