摘要
目的:采用两泵一阀的在线固相萃取-高效液相色谱技术建立一种能够快速检测食品中胡萝卜素的方法,避免了胡萝卜素在复杂前处理过程中的损失。方法:在线固相萃取柱PLRP-S(2.1 mm×12.5 mm)液相分离色谱柱PAH(2.1 mm×100 mm,1.8μm);固相萃取泵流动相,水+甲醇,液相分离流动相,甲醇+二氯甲烷;检测波长450 nm。结果:该方法在0.10~2.00μg/mL保持良好的线性,方法的检出限和定量限均能够满足检测需求。α-胡萝卜素回收率为92.0%~105.4%,β-胡萝卜素回收率91.1%~107.2%。结论:该方法利用两泵一阀的在线固相萃取技术,能高效、快速检测食品中胡萝卜素,检测效率高,前处理提取效率好,方法能够满足检测需要。
【Objective】The experiment established a rapid detection method for carotene in food using online solid-phase extraction and high-performance liquid chromatography technology with two pumps and one valve,which minimized the loss of carotene during the complex sample preparation.【Method】The solid-phase extraction column PLRP-S(2.1 mm×12.5 mm)and the liquid chromatography column PAH(2.1 mm×100 mm,1.8μm)were used in this method.The mobile phase for online solid-phase extraction was water and methanol,and for liquid chromatography separation was methanol and dichloromethane.In this methodthe detection wavelength of 450 nm was applied.【Result】The regression equation of the standard curve maintained good linearity when the concentration ranging from 0.10 to 2.00μg/mL.Also,both the limit of detection and the limit of quantification met requirements,and the recovery rate ofα-carotene was between 92.0%and 105.4%,the recovery rate ofβ-carotene was between 91.1%and 107.2%.【Conclusion】This method utilized an online solid-phase extraction system with two pumps and one valve to efficiently and quickly test the content of carotene in food.The advantages are high detection efficiency and better extraction efficiency during sample preparation,so that the method can meet current detection demands.
作者
俞淑
庞贝妮
林毅侃
YU Shu;PANG Bei-ni;LIN Yi-kan(Shanghai Institute of Quality Inspection and Technical Research,National Institute of Quality Inspection and Research on Product in Shanghai/Key Laboratory of Milk and Dairy Products Detection and Monitoring Technology for State Market Regulation,Shanghai 200233,China)
出处
《中国食物与营养》
2024年第4期49-52,共4页
Food and Nutrition in China
