摘要
为了明确引起四川省广汉市鱼腥草新型叶斑及茎斑类病害的病原菌种类,为该病的科学防治提供理论依据,对采自广汉市的鱼腥草病茎叶通过组织消毒分离、离体接种、形态学观察与基于核糖体转录间隔区(ITS)、延长因子(tef1)、钙调蛋白(cal)和组蛋白(his3)基因的分子鉴定进行病原菌的分离鉴定。结果表明,病原菌菌丝呈灰白色到浅褐色,其分生孢子器可产生纺锤形到椭圆形的α型分生孢子以及丝状或钩状β型分生孢子。多基因联合系统发育分析表明,病原菌在大豆间座壳(Diaporthe sojae)和菜豆间座壳(D.phaseolorum)分枝外单独聚为一枝,并且3枝一起聚为一个大枝,因此建议成立一个新种—鱼腥草间座壳(D.houttuynia)来容纳此类病菌。结合形态学和分子鉴定结果,将引起广汉市鱼腥草新型叶斑及茎斑类病害的病原菌鉴定为鱼腥草间座壳(D.houttuynia)。
To identify the pathogen species causing a noval desease of leaf spot and stem spot of Houttuynia cordata Thunb.in Guanghan City,Sichuan Province,and provides theoretical basis for the scientific management of this disease,the leaves and stems of diseased H.cordata were collected from Guanghan City,and the pathogen was isolated and identified by tissue isolation,pathogenicity testing,morphological characterization,and molecular identification based on ITS,tef1,cal and his3 gene sequences.The results indicate that the mycelium of the pathogen is gray-white to light brown.The conidiomata,which are produced in the late growth stage,can produce two types of conidia:fusiform to oval-shapedα-type conidia and filamentous or hook-shapedβ-type conidia.Multiple gene cluster analysis showed that the pathogen formed an individual branch adjacent to the Diaporthe Sojae and D.phaseolorum branches,and formed a big branch with these two branches in the phylogenetic tree,and a new species named D.houttuynia was suggested to establish to accommodate this pathogen.According to morphology and molecular identification,the pathogen causing the noval desease of leaf spot and stem spot of H.cordata in Guanghan City was identified as D.houttuynia.
作者
张重梅
谢杰
张蕾
杨潇湘
黄小琴
邓越
胡孜进
余垚颖
鲜赟曦
马凡
刘勇
ZHANG Zhongmei;XIE Jie;ZHANG Lei;YANG Xiaoxiang;HUANG Xiaoqin;DENG Yue;HU Zijin;YU Yaoying;XIAN Yunxi;MA Fan;LIU Yong(Institute of Plant Protection,Sichuan Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management in Southwest Agriculture Crops,Ministry of Agriculture and Rural Affairs,Chengdu 610066;Agriculture and Rural Bureau of Wusheng County,Wusheng,Sichuan 638400)
出处
《中国农学通报》
2024年第12期119-125,共7页
Chinese Agricultural Science Bulletin
关键词
鱼腥草
叶斑病
茎斑病
分离鉴定
鱼腥草间座壳
Houttuynia cordata
leaf spot
stem spot
isolation and identification
Diaporthe houttuynia
