摘要
为探究低C/N城镇污水处理在无外加碳源的情况下实现深度脱氮除磷的效能,试验以实际城镇生活污水为处理对象,搭建了日处理规模为100 m^(3)·d^(-1)的A^(2)/O-BCO前置反硝化除磷中试,并从中试快速启动、工况调整与优化、微生物群落分析等角度进行研究.结果表明,通过先在A^(2)/O单元与BCO单元中分别富集聚磷菌与硝化菌,再增加硝化液回流以富集反硝化除磷菌(DPAOs)的方式,可实现A^(2)/O-BCO前置反硝化除磷中试的快速启动.A^(2)/O单元厌氧/缺氧/好氧容积比调整为3∶5∶1,硝化液回流比设定为250%时,缺氧段对磷去除率的平均值达到95%,平均出水PO_(4)^(3-)-P浓度小于0.2 mg·L^(-1),出水TN小于11 mg·L^(-1).通过分子生物学分析发现,除磷菌(PAOs)与硝化菌丰度显著提高,Candidatus Accumulibacter的相对丰度从原始种泥的2%增加到14%,已被证实具有反硝化聚磷功能的Dechloromonas的相对丰度从原始种泥的2%增加到7%,BCO单元中的生物膜上,Nitrosomonas的相对丰度为5.91%,Nitrospira的相对丰度为7.58%.
In order to achieve advanced nitrogen and phosphorus removal in low C/N ratio urban sewage without the addition of external carbon source,this study focused on the treatment of actual urban domestic wastewater,and constructed a pilot-scale A^(2)/O-BCO system(pre-denitrification phosphorus removal)with a daily treatment capacity of 100 m3⸱d-1.The research encompassed aspects including rapid startup of the pilot system,operational adjustments and optimization,and analysis of the microbial community.The rapid startup of the pilot scale A^(2)/O-BCO system was achieved by first enriching phosphorus-accumulating organisms(PAOs)in the A^(2)/O unit and nitrifying bacteria in the BCO unit separately,then increasing the return flow of nitrified effluent to enrich denitrifying phosphorus-accumulating organisms(DPAOs).In the A^(2)/O unit,the anaerobic/anoxic/aerobic volume ratio was adjusted to 3∶5∶1,and when the return flow ratio of nitrified effluent was set at 250%,the average phosphorus removal efficiency in the anoxic stage reached 95%,and the average effluent PO_(4)^(3-)-P and TN(total nitrogen)concentrations were less than 0.2 and 11 mg·L^(-1),respectively.Molecular biology analysis revealed a significant increase in the abundance of PAOs and nitrifying bacteria.The relative abundance of Candidatus Accumulibacter increased from 2%in the seed sludge to 14%.And Dechloromonas,a confirmed organism with denitrifying and phosphorus-accumulating functions,increased from 2%to 7%.In the biofilm of the BCO unit,Nitrosomonas accounted for 5.91%of the microbial community,and Nitrospira accounted for 7.58%.
作者
周庆国
苏健
陈东旭
彭永臻
ZHOU Qingguo;SU Jian;CHEN Dongxu;PENG Yongzhen(School of Civil Engineering,Southwest Jiaotong University,Chengdu 610031;Research Center for Water Treatment Technology,CRCC Development Group Co.,Ltd.,Beijing 100043;National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering,Beijing University of Technology,Beijing 100124)
出处
《环境科学学报》
CAS
CSCD
北大核心
2024年第3期13-22,共10页
Acta Scientiae Circumstantiae
基金
中铁建发展集团有限公司科技研发计划项目。
关键词
低C/N城镇污水
前置反硝化除磷
中试规模
无外加碳源
快速启动
low C/N urban wastewater
pre-denitrifying phosphorus removal
pilot-scale
no external carbon source
rapid start-up
