摘要
目的为探索呼吸道合胞病毒(respiratory syncytial virus,RSV)感染对慢性鼻窦炎伴鼻息肉(CRSwNP)和对照黏膜来源的人鼻黏膜上皮细胞(human nasal epithelial cells,hNECs)物理屏障关键分子的影响。方法不同感染复数(multiplicity of infection,MOI)(0.1和0.3)的RSV分别感染鼻息肉(n=21)和对照黏膜(n=9)的hNECs 24 h和48 h后,提取总RNA,逆转录成cDNA后,采用实时荧光定量PCR检测ZO-1、ZO-2、Claudin-1、Claudin-4、Occludin、E-cadherin和N-cadherin的基因表达变化。结果RSV感染hNECs后,ZO-1、ZO-2、Claudin-1、Claudin-4、Occludin、E-cadherin和N-cadherin的基因表达水平均下降,但只在鼻息肉来源的hNECs中存在统计学差异(P<0.05)。RSV感染嗜酸性粒细胞型CRSwNP(eosinophilic CRSwNP,ECRSwNP)与非嗜酸性粒细胞型CRSwNP(nonECRSwNP)的hNECs相比,无显著差异。结论RSV感染可破坏鼻黏膜上皮屏障,与对照组相比CRSwNP组受RSV感染影响更重,且ECRSwNP组与nonECRSwNP组无显著差异。
OBJECTIVE To explore the effect of respiratory syncytial virus(RSV)infection on the expression of key factors in the epithelial barrier of the human nasal epithelial cells(hNECs)derived from patients with chronic rhinosinusitis with nasal polyps(CRSwNP)and normal control mucosa.METHODS RSV with different multiplicity of infection(MOI)(0.1 and 0.3)infected hNECs derived from patients with CRSwNP(n=21)and normal control mucosa(n=9)for 24 h and 48 h,respectively.To detect the gene expression ZO-1,ZO-2,Claudin-1,Claudin-4,Occludin,E-cadherin and N-cadherin,total RNA was extracted and reverse transcribed into cDNA for real-time fluorescence quantification PCR.RESULTS The relative expression level of ZO-1,ZO-2,Claudin-1,Claudin-4,Occludin,E-cadherin and N-cadherin were decreased in hNECs post RSV infection.However,there was a statistical difference only in hNECs derived from CRSwNP(P<0.05).There was no significant difference in hNECs infected with RSV between eosinophilic CRSwNP(ECRSwNP)and non-eosinophilic CRSwNP(nonECRSwNP).CONCLUSION RSV infection could disrupt the epithelial barrier of the nasal mucosa,and patients with CRSwNP are more severely affected by RSV infection compared to healthy controls.The impact of RSV infection on mucosa between ECRSwNP group and nonECRSwNP group was no significant difference.
作者
康子卿
杨婧兴
王向东
蔡超
KANG Ziqing;YANG Jingxing;WANG Xiangdong;CAI Chao(Department of Otolaryngology Head and Neck Surgery,Beijing You'an Hospital,Capital Medical University,Beijing,100069,China;Department of Otolaryngology Head and Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Beijing Institute of Otolaryngology,Key Laboratory of Otolaryngology Head and Neck(Capital Medical University),Ministry of Education,Beijing Key Laboratory of Nasal Diseases,Beijing,100005,China)
出处
《中国耳鼻咽喉头颈外科》
CSCD
2024年第1期31-35,共5页
Chinese Archives of Otolaryngology-Head and Neck Surgery
基金
北京市自然科学基金(7222024)
北京市科技新星计划(20220484226)
公益发展与改革项目(2019-10)
北京市高层次公共卫生技术人才计划(领军人才01-08、学科骨干2022-3-002)。
关键词
鼻窦炎
鼻息肉
呼吸道合胞病毒
上皮细胞
鼻粘膜
紧密连接部
人鼻黏膜上皮细胞
Sinusitis
Nasal Polyps
Respiratory Syncytial Viruses
Epithelial Cells
Nasal Mucosa
Tight Junctions
human nasal epithelialcells
