摘要
为实现显微镜可实现快速检测的同时,拥有较好的便携性,提出设计一款小型化显微镜,从软硬件的设计上,更是针对研发成本和便携性进行了多方面考量,借助感光性较好的网络相机CMOS主板作为成像器件,闭环步进电机平移台作为载物台,利用UG软件自主进行机械结构的设计,最终使得整个尺寸控制在5 cm*10 cm*15 cm范围内,相对市面售卖的显微镜,体积缩小约80%;更是利用3D打印技术来降低成本;设计配套的交互软件,界面美观且人机交互友好。为加快图像的对焦速度,将基于图像信息的自动对焦算法嵌入到自研显微镜中。通过几种清晰度评价函数对同一组图像序列进行实际的评估对比分析,Laplacian函数对于单张图像的计算速度较快;最终针对平移台进行了稳定性控制实验,结果得知其精度误差最高可达0.03 mm。
To achieve fast detection and good portability in microscopic imaging,a miniaturized microscope was designed with consideration for both research and development costs and portability.The design included both hardware and software components.A network camera CMOS mainboard with good sensitivity was used as the imaging device,while a closed-loop stepper motor translation stage was used as the sample holder.Mechanical structure design was conducted autonomously using UG software,resulting in a total size within the range of 5 cm×10 cm×15 cm.Compared to commercially available microscopes,this represents a volume reduction of approximately 80%.3D printing technology was also used to reduce costs.An interactive software program was designed to provide an aesthetically pleasing and user-friendly interface.To speed up the autofocus process,an image-based autofocus algorithm was embedded into the self-developed microscope.Several sharpness evaluation functions were used to compare and analyze the same set of image sequences.The Laplacian function was found to be faster for computing the sharpness of a single image.Finally,stability control experiments were conducted on the translation stage,and the results showed that the accuracy error could reach a maximum of 0.03 mm.
作者
文显琼
赵英然
WEN Xianqiong;ZHAO Yingran(School of Intelligent Science and Information Engineering,Xi’an Peihua University,Xi’an 710125,China;School of Electronic Information Engineering,Xi’an Technological University,Xi’an 710021,China)
出处
《自动化与仪器仪表》
2023年第11期259-262,共4页
Automation & Instrumentation
基金
陕西省教育厅专项科研项目(22JK0493)。
