摘要
目的 研究紫草素联合半导体激光对牙釉质表面脱矿的抑制作用。方法 收集2021年12月-2022年7月在新疆医科大学第一附属医院(附属口腔医院)就诊的120位15~20岁因正畸减数拔牙的新鲜前磨牙或第三磨牙90颗,用低速金刚石锯将其切割制备成5 mm×5 mm×3 mm釉质块,选择硬度值在310~350 HV范围内的牙釉质块样本。随机分类法将其分为对照组、紫草素组、激光组、激光联合氟化钠组、氟化钠组、激光联合紫草素组,每组15颗。每组进行3 d的脱矿实验(期间每隔12 h各组按条件给予干预)后,采用显微硬度仪测定各组表面硬度;采用原子力显微镜测定各组脱矿深度、表面粗糙度和表面形貌;采用X射线能谱仪测定各组表面形貌和表面元素变化。结果 与对照组、激光组、紫草素组比较,激光联合紫草素组显微硬度值增大,差异有统计学意义(P<0.05);与激光联合氟化钠组比较,激光联合紫草素组显微硬度值减小,差异有统计学意义(P<0.05);与氟化钠组比较,激光联合紫草素组显微硬度值差异无统计学意义(P>0.05)。与对照组、激光组、紫草素组比较,激光联合紫草素组表面粗糙度和脱矿深度减小,差异有统计学意义(P<0.05);与激光联合NaF组比较,激光联合紫草素组表面粗糙度和脱矿深度增大,差异有统计学意义(P<0.05);NaF组比较,激光联合紫草素组表面粗糙度和脱矿深度差异无统计学意义(P>0.05)。与对照组比较,激光联合紫草素组表面钙磷元素摩尔比值增大,差异有统计学意义(P<0.05);与激光联合NaF组、NaF组比较,激光联合紫草素组表面钙磷元素摩尔比值减小,差异有统计学意义(P<0.05);与激光组、紫草素组比较,激光联合紫草素组表面钙磷元素摩尔比值差异无统计学意义(P>0.05)。结论 紫草素联合半导体激光对牙釉质表面脱矿有明显的抑制作用。
Objective To research the effects of Shikonin combined with semiconductor laser on enamel surface demineralization by in vitro experiments.MethodsIn this experiment,90 fresh premolars or third molars of 120 patients aged 15-20 years old attending the hospital for orthodontic minus extractions from December 2021 to July 2022 were uniformly collected,and the patients were cut and prepared into 5 mm×5 mm×3 mm enamel blocks using a low-speed diamond saw,and hardness values in the range of 310~350 HV were selected for the enamel block samples.Demineralization experiment:the patients were divided into control no treatment group,Shikonin group,laser group,laser+NaF group,NaF group and laser+Shikonin group according to random classification method with 15 samples in each group.Each group was intervened by different methods for 3 days of demineralization experiments(during which interventions were made every 12 hours according to each subgroup),and then the surface hardness of the samples was detected by microhardness tester;the surface demineralization depth and surface roughness,surface morphology of the samples were evaluated by atomic force microscopy(AFM);and the surface morphology and surface elemental changes of the samples were studied and analyzed by X-ray energy spectrometry(EDS).ResultsCompared with the control group,laser group,and shikonin group,the microhardness value of the laser combined with shikonin group was increased,and the difference was statistically significant(P<0.05).Compared with the laser combined with sodium fluoride group,the microhardness value of the laser combined with shikonin group was decreased,and the difference was statistically significant(P<0.05).Compared with the sodium fluoride group,there was no statistically significant difference in microhardness values between the laser combined with shikonin group(P>0.05).Compared with the control group,laser group,and shikonin group,the surface roughness and demineralization depth of the laser combined with shikonin group was decreased,and the difference was statistically significant(P<0.05).Compared with the laser combined with NaF group,the laser combined with shikonin group showed an increase in surface roughness and demineralization depth,and the difference was statistically significant(P<0.05).Compared with the NaF group,there was no statistically significant difference in surface roughness and demineralization depth between the laser combined with shikonin group(P>0.05).Compared with the control group,the molar ratio of surface calcium and phosphorus elements in the laser combined with purpurin group was increased,and the difference was statistically significant(P<0.05).Compared with the laser combined with NaF group and NaF group,the molar ratio of surface calcium and phosphorus elements in the laser combined with shikonin group was decreased,and the difference was statistically significant(P<0.05).Compared with the laser group and the shikonin group,there was no statistically significant difference in the molar ratio of surface calcium and phosphorus elements between the laser combined with shikonin group(P>0.05).ConclusionShikonin combined with semiconductor laser has a significant inhibitory effect on tooth enamel surface demineralization.
作者
范文献
范译丹
赵今
李新尚
FAN Wenxian;FAN Yidan;ZHAO Jin;LI Xinshang(The First Affiliated Hospital of Xinjiang Medical University(Affiliated Stomatology Hospital),the First Affiliated Hospital of Xinjiang Medical University(Affiliated Stomatology Hospital),Urumqi 830054,China;Department of Restoration and Planting,the First Affiliated Hospital of Xinjiang Medical University(Affiliated Stomatology Hospital),the First Affiliated Hospital of Xinjiang Medical University(Affiliated Stomatology Hospital),Urumqi 830054,China;Department of Oral Emergency Comprehensive,the First Affiliated Hospital of Xinjiang Medical University(Affiliated Stomatology Hospital),Urumqi 830054,China)
出处
《新疆医科大学学报》
CAS
2023年第12期1612-1617,1622,共7页
Journal of Xinjiang Medical University
基金
中华口腔医学会西部临床科研基金项目(CSA-W2021-02)。
关键词
牙釉质
半导体激光
紫草素
脱矿
原子力显微镜
体外研究
微观形貌
dental enamel
semiconductor laser
shikonin
demineralization
atomic force microscopy
in vitro study
microstructure
