摘要
目的 :探讨吗啡对神经系统细胞的影响及作用机理。方法 :应用基因克隆技术克隆大鼠增殖细胞核抗原 ( PCNA)的 c DNA基因片段 ,并应用逆转录 PCR技术研究了吗啡对大鼠胶质细胞瘤C6细胞 PCNA基因表达的影响。结果 :0 .1、1 .0 mg· L-1吗啡作用于大鼠 C6细胞 2 4 h后 ,PCNAm RNA相对含量明显下降 ,且剂量越高 PCNA m RNA相对含量越低。结论 :吗啡对正常生长的大鼠 C6细胞的增殖具有一定的抑制作用 ,其机理可能是通过抑制大鼠 C6细胞 PCNA的转录过程 ,使 PCNA转录水平降低 。
Objective:To study the effect of morphine on nerve system cells and its mechanism. Methods: Using gene cloning technique to get the recombinant of PCNA gene fragment; Using RT PCR method to study the effects of morphine on the level of PCNA gene transcript in C6 rat glioma cells treated with different doses of morphine.Results: The level of PCNA gene transcript in C6 rat glioma cells was down regulated after treated with 0.1,1.0 mg·L -1 morphine for 24 hours in a dose dependent way. Conclusion:Morphine has a certain inhibitory effect on the proliferation of cultured C6 cells. Its mechanism should be that morphine inhibits the transcript of PCNA, thereby inhibits the anabolism of nucleic acid.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2002年第6期594-596,共3页
Journal of Jilin University：Medicine Edition
