摘要
为消除铬天青S分光光度法测定臭豆腐中铝时铁带来的干扰,采用氢氧化钠溶液对铁和铝进行分离,并对方法进行优化。结果表明:不对臭豆腐消解液进行处理,采用铬天青S分光光度法测定其铝含量,当溶液铝含量一定时,溶液中铁含量越高,测得的结果越比真实值偏高,当溶液铁含量较高且一定,铝含量越高,测得的结果越接近真实值。采用优化后的方法对含有大量铁的臭豆腐消解液进行处理后,用铬天青S分光光度法测定其铝,测定结果与电感耦合等离子体质谱法测定的结果都没有显著性差异(P>0.05);而铬天青S分光光度法测定未处理消解液的铝,其结果都显著高于(P<0.05)电感耦合等离子体质谱法测定的结果。因此,通过本方法对含有大量铁的臭豆腐消解进行处理后,采用铬天青S分光光度法能够准确测定样品中铝。
In order to eliminate iron interference in the determination of aluminum with Chrome Azurol S Spectrophotometry,sodium hydroxide was used to separate iron and aluminum in the solution.The sodium hydroxide separation method was optimized.Chrome Azurol S Spectrophotometry was used to determine the aluminum in the untreated stinky tofu digestion solution.When certain aluminum content in the solution remained constant,the higher the iron content in the solution,the higher the measured result compared to the true value.When iron content was high in the solution and remain constant,the higher the aluminum content was,the closer the result was to the true value.After the digestion solution of stinky tofu containing a large amount of iron was treated with the optimized method,its aluminum was determined by Chrome Azurol S Spectrophotometry,and there was no significant difference between the determination results and those of inductively coupled plasma mass spectrometry(P>0.05).The measurement results of aluminum in untreated digestion solution by Chrome Azurol S Spectrophotometry were significantly higher than those measured by ICP-MS(P<0.05).Therefore,after a large amount of iron in the stinky tofu was digested,aluminum in the sample could be accurately determined by Chrome Azurol S Spectrophotometry.
作者
王小平
刘忠莹
张定秋
钟洋
陆阳
朱敏敏
郑红毅
王鑫
WANG Xiaoping;LIU Zhongying;ZHANG Dingqiu;ZHONG Yang;LU Yang;ZHU Minmin;Zheng Hongyi;WANG Xin(Sichuan Institute of Food Inspection,Chengdu 610097;National Key Laboratory of Market Supervision(Baijiu Supervising Technology),Chengdu 610097)
出处
《中国食品添加剂》
CAS
北大核心
2023年第11期168-173,共6页
China Food Additives
基金
四川省科技厅(2020YFH0157)。
关键词
铝
铁
铬天青S
分光光度法
干扰消除
aluminum
iron
chrome azurol S
spectrophotometry
interference
elimination
