摘要
为探究黑玉米粒多糖的最佳提取工艺及其体外抗氧化活性。本研究以黑玉米粒为原材料,采用超声辅助法提取黑玉米粒多糖,探究超声功率、料液比、提取时间、提取温度、提取次数对黑玉米粒多糖得率的影响,在此基础上结合响应面法优化黑玉米粒多糖提取工艺,并通过测定DPPH·、ABTS^(+)·、·OH的清除率来评价黑玉米粒多糖的体外抗氧化能力。结果表明,在料液比1:20(g/mL)、提取温度74℃、提取时间60 min、提取3次的条件下,黑玉米粒多糖得率最高,可达41.09%±0.59%。黑玉米粒多糖对DPPH·、ABTS^(+)·、·OH清除率的半数清除浓度(IC_(50))分别为1.959、1.529、0.3554 mg/mL,且清除能力在一定范围内与浓度呈量效关系,具有较强的体外抗氧化能力,为其深入研究及开发利用提供重要依据。
In order to explore the optimum extraction process of polysaccharide and antioxidant activity in vitro in black corn kernel.In this study,black corn kernel was used as raw material,ultrasonic-assisted extraction was applied to extract polysaccharides from black corn kernel.To explore the effects of ultrasonic power,solid-liquid ratio,extraction time,temperature and frequency on the yield of polysaccharide.The extraction process of polysaccharide from black corn kernel was optimized by response surface methodology.Besides,the antioxidant activity of the polysaccharide was investigated by measuring its scavenging ability on DPPH·,ABTS^(+)·,and·OH.The results showed that the extraction yield of polysaccharide from black corn kernel could reach up to 41.09%±0.59%,in these conditions:The solid-liquid ratio was 1:20 g/mL,the extraction temperature was 74℃,the extraction time was 60 min and the extraction frequency was 3 times.The IC_(50)values of scavenging rates on DPPH·,ABTS^(+)·and·OH were 1.959,1.529 and 0.3554 mg/mL,respectively.Moreover,the scavenging rates showed a certain dose-effect relationship with the sample concentration,indicating that the polysaccharide had a strong antioxidant activity,thus providing a theoretical basis for further research and utilization.
作者
王燕
段雪伟
张敏君
杨慧文
刘冰
由天辉
WANG Yan;DUAN Xuewei;ZHANG Minjun;YANG Huiwen;LIU Bing;YOU Tianhui(School of Nursing,Guangdong Pharmaceutical University,Guangzhou 510006,China;School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China)
出处
《食品工业科技》
CAS
北大核心
2023年第22期191-200,共10页
Science and Technology of Food Industry
基金
广东省教育委员会项目(2021KCXTD056)。
关键词
黑玉米粒
多糖
超声波
提取工艺优化
抗氧化活性
black corn kernel
polysaccharide
ultrasonic
optimization of extraction process
antioxidant activity
