摘要
为研究不同光周期对肉鸡胸腺和脾脏褪黑激素受体(Mel1a、Mel1b、Mel1c)表达的影响,阐明光周期影响肉鸡免疫功能的可能途径,本试验选用0日龄雄性817肉鸡45只,按照光照∶黑暗为8 h∶16 h、12 h∶12 h、16 h∶8 h三种不同的光周期随机分为Ⅰ、Ⅱ、Ⅲ组。饲养2周后取肉鸡胸腺和脾脏组织,测定其器官指数,采用反转录聚合酶链式反应(RT-PCR)方法检测其褪黑激素受体Mel1a、Mel1b和Mel1c基因的相对表达水平。结果显示,与Ⅱ组和Ⅲ组相比,Ⅰ组胸腺指数分别提高了16.13%(P<0.05)和22.03%(P<0.05),脾脏指数分别提高了19.40%(P>0.05)和21.21%(P<0.05),胸腺Mel1a基因相对表达水平分别升高了27.25%(P<0.05)和88.56%(P<0.05),脾脏Mel1a基因相对表达水平分别升高了11.53%(P<0.05)和19.08%(P<0.05),胸腺Mel1b基因相对表达水平分别升高了15.59%(P>0.05)和30.65%(P<0.05),脾脏Mel1b基因相对表达水平分别升高了18.07%(P>0.05)和40.11%(P<0.05),胸腺Mel1c基因相对表达水平分别升高了17.79%(P<0.05)和70.14%(P<0.05),脾脏Mel1c基因相对表达水平分别升高了34.05%(P<0.05)和63.77%(P<0.05)。结果表明,短光照可提高肉鸡免疫器官指数和褪黑激素受体基因的相对表达水平。
To investigate the effects of different photoperiods on the expression of melatonin receptors(Mel1a,Mel1b,Mel1c)in thymus and spleen of broilers,and elucidate the possible pathways through which photoperiods influence the immune function of broilers,this study selected 45 male 817 broilers at 0-day-old and randomly divided them into three groups(GroupⅠ,GroupⅡ,GroupⅢ)according to the photoperiods of 8 h light∶16 h dark,12 h light∶12 h dark and 16 h light∶8 h dark,respectively.After two weeks of feeding,the thymus and spleen tissue were collected,and the organ indexes were determined.The relative expression levels of Mel 1 a,Mel 1 b and Mel 1 c genes were detected by reverse transcription-polymerase chain reaction(RT-PCR).The results showed that compared with GroupⅡand GroupⅢ,the thymus index of GroupⅠwas increased by 16.13%(P<0.05)and 22.03%(P<0.05),and the spleen index increased by 19.40%(P>0.05)and 21.21%(P<0.05),respectively.The relative expression levels of Mel 1 a gene in the thymus increased by 27.25%(P<0.05)and 88.56%(P<0.05),and in the spleen increased by 11.53%(P<0.05)and 19.08%(P<0.05)in GroupⅠcompared with GroupⅡand GroupⅢ,respectively.The relative expression levels of Mel 1 b gene in the thymus increased by 15.59%(P>0.05)and 30.65%(P<0.05),and in the spleen increased by 18.07%(P>0.05)and 40.11%(P<0.05)in GroupⅠcompared with GroupⅡand GroupⅢ,respectively.The relative expression levels of Mel 1 c gene in the thymus increased by 17.79%(P<0.05)and 70.14%(P<0.05),and in the spleen increased by 34.05%(P<0.05)and 63.77%(P<0.05)in GroupⅠcompared with GroupⅡand GroupⅢ,respectively.The results indicate that short photoperiods can increase the immune organ indexes and relative expression levels of melatonin receptor genes of broilers.
作者
张利卫
刘冠慧
白欣洁
王军
张曼
赵源
赵亚选
宋予震
ZHANG Li-wei;LIU Guan-hui;BAI Xin-jie;WANG Jun;ZHANG Man;ZHAO Yuan;ZHAO Ya-xuan;SONG Yu-zhen(College of Veterinary Medicine,Henan University of Animal Husbandry and Economy,Zhengzhou 450046,China;School of Life Sciences and Food Engineering,Hebei University of Engineering,Handan 056038,China;Department of Animal Husbandry and Veterinary Medicine,Beijing Vocational College of Agriculture,Beijing 102442,China)
出处
《中国兽医杂志》
CAS
北大核心
2023年第8期12-17,共6页
Chinese Journal of Veterinary Medicine
基金
河南省自然科学基金项目(222300420451)
河南牧业经济学院博士科研启动基金项目(2018HNUAHEDF030)
国家自然科学基金青年科学基金项目(31802150)
北京市自然科学基金项目(6214035)
河南省高等学校教学名师项目(教高[2020]434号)
河南牧业经济学院校级教改项目(2021-XJLX-144)
河南省高等学校重点科研项目(20A230003)。
关键词
光周期
肉鸡
胸腺
脾脏
褪黑激素受体
photoperiod
broiler
thymus
spleen
melatonin receptor
