摘要
目的:通过核因子NF-E2相关因子(Nrf2)/血红素氧合酶-1(HO-1)信号通路研究丁苯酞治疗糖尿病脑小血管病大鼠的作用机制。方法:将建模成功的糖尿病脑小血管病大鼠随机分为丁苯酞治疗组和模型组,行水迷宫实验,取材后石蜡切片染色、脱水封片,显微镜观察大鼠大脑皮质的组织学结构及该区域神经元细胞的组织病理学改变;应用Western blot法检测脑组织Nrf2/HO-1信号通路中Nrf2和HO-1蛋白表达。结果:水迷宫实验d 1~d 4,与模型组相比,丁苯酞治疗组大鼠定位航行实验逃避潜伏期逐渐缩短。脑组织镜下观察,与模型组相比,丁苯酞治疗组大鼠脑组织细胞排列规则、细胞核饱满圆润、固缩现象减少。Western blot法检测发现,与模型组相比,丁苯酞治疗组大鼠脑组织中Nrf2和HO-1蛋白表达增高。结论:丁苯酞治疗组大鼠脑组织中Nrf2和HO-1增加,表明丁苯酞可能通过上调抗氧化应激蛋白的表达发挥脑保护作用。
Objective:To study the mechanism of action of DL-3-N-butylphthalide(NBP) on cerebral small vessel disease in diabetic rats based on Nrf2/HO-1 signaling pathway.Methods:The successfully modeled diabetes rats with cerebrovascular disease were randomly divided into NBP treatment group and model group.The water maze test was carried out.After taking the materials,paraffin sections were stained,dehydrated and sealed.The histological structure of the rat cerebral cortex and the histopathological changes of neurons in this area were observed under a microscope.The expressions of Nrf2 and HO-1 protein in Nrf2/HO-1 signal pathway of brain tissue were detected by Western blot.Results:Compared with the model group,the escape latency of the rats in the NBP-treated group was shortened gradually on the 1st-4th day in the water maze test.Compared with the model group,the cells of brain tissue in the NBP-treated group were arranged regularly,the nuclei were full and round,and the pyknosis was decreased.Western blot analysis showed that Nrf2 and HO-1 protein expression in the brain tissue of rats treated with NBP was higher than that of the model group.Conclusion:Nrf2 and HO-1 were increased in the brain tissue of NBP-treated rats,and NBP may play a protective role in the brain by up-regulating the expression of anti-oxidative stress protein.
作者
牟肖莉
王波
赵娟
于金凤
MOU Xiao-li;WANG Bo;ZHAO Juan;YU Jin-feng(Yantaishan Hospital,Yantai 264000,China)
出处
《中国新药杂志》
CAS
CSCD
北大核心
2023年第14期1467-1471,共5页
Chinese Journal of New Drugs
