摘要
目的研究2型糖尿病(T2DM)患者浓缩生长因子(CGF)纤维蛋白凝胶中生长因子及其含量。方法选取胰岛素降糖治疗下需拔牙及种植的T2DM患者,根据糖化血红蛋白(HbA1c)结果分组后,抽取外周血,离心获得CGF。用酶联免疫吸附试验(ELISA)双抗体夹心法测试TGF-β1、EGF、OPG、PDGF-BB、VEGF、IGF-1、BMP-2、bFGF、TNF-α和IL-1β10种生长因子及其含量。结果胰岛素控制下T2DM患者的CGF凝胶中含有与健康对照组相同的10种生长因子;胰岛素控制良好和中等的T2DM患者的OPG含量与健康对照组比较,差异无统计学意义(P>0.05),与控制不良组比较,差异有统计学意义(P<0.05);不同分组的T2DM患者的部分生长因子(TGF-β1、EGF)含量较健康对照组低(P<0.05),部分生长因子(PDGF-BB、VEGF、IGF-1、BMP-2、bFGF、TNF-α和IL-1β)含量和健康对照组比较,差异无统计学意义(P>0.05)。结论胰岛素控制下T2DM患者的CGF纤维蛋白凝胶中仍含多种生长因子,可刺激细胞增殖与迁移分化,具有改善成骨质量和促进软硬组织愈合,再生与修复潜力强等功能。
Objective To study the growth factors and its contents in the concentrated growth factor(CGF)fibrin gel for type 2 diabetes mellitus(T2DM).Methods T2DM patients who needed tooth extraction and implantation under insulin hypoglycemic were selected and grouped according to the hemoglobin A 1 c(HbA1c)results,peripheral blood was drawn and centrifuged to obtain CGF.In samples,TGF-β1,EGF,OPG,PDGFBB,VEGF,IGF-1,BMP-2,Bfgf,TNF-αand IL-1βwere tested by enzyme-linked immunosorbent assay(ELISA).Results The CGF gels from insulin-controlled T2DM patients contained the same 10 growth factors as those from healthy controls.There was no significant difference in the content of OPG in T2DM patients with good and moderate insulin control compared with the healthy control group(P>0.05).There were significant differences from the poorly controlled groups(P<0.05).The content of partial growth factors(TGF-1,EGF)of T2DM patients in different groups was lower than that of healthy controls(P<0.05).There was no significant difference in the contents of some growth factors(PDGF-BB,VEGF,IGF-1,BMP-2,bFGF,TNF-αand IL-1β)between the observation group and healthy control group(P>0.05).Conclusion The CGF fibrin gel of insulin-controlled T2DM patients contains a variety of growth factors,which can stimulate cell proliferation,migration and differentiation,improve bone formation quality,and promote soft and hard tissue healing,with strong regeneration and repair potential functions.
作者
袁艳波
阮兆娟
曹良菊
杨万娟
蒋愉
周雪
YUAN Yanbo;RUAN Zhaojuan;CAO Liangju;YANG Wanjuan;JIANG Yu;ZHOU Xue(Dept.of Stomatology,Kunming 1st People’s Hospital,Kunming Yunnan 650011;Dept.of Endocrinology,The 92nd Hospital of the Joint Logistic Support Force of the PLA,Kunming Yunnan 650032,China)
出处
《昆明医科大学学报》
CAS
2023年第7期125-129,共5页
Journal of Kunming Medical University
基金
云南省教育厅科学研究基金资助项目(2022J0209)
昆明市卫生健康委员会卫生科研课题项目(2022-08-04-001)
昆明市卫生科技人才培养项目(2022-SW-34)。
关键词
2型糖尿病
浓缩生长因子
活性因子
ELISA
Type 2 diabetes mellitus
Concentrated growth factor
Active factors
ELISA
