摘要
以BY-2烟草悬浮细胞为实验材料,探究在不同磷酸盐供应水平下细胞内ATP(iATP)、细胞外ATP(eATP)水平的变化以及NO在其中的调节作用.结果表明,随着磷酸盐供应水平的增加,iATP水平呈现上升趋势;伴随着iATP的上升,eATP也呈现相似的变化.与ATP的变化不同,随着磷酸盐供应水平的增加NO水平呈现下降趋势.在正常水平磷酸盐供应下,NO供应剂可增加iATP和eATP水平;NO合成抑制剂降低了iATP和eATP水平.在较低和较高水平的磷酸盐供应下,NO供应剂和合成抑制剂对于iATP和eATP水平的影响呈现与正常水平磷酸盐供应下不同的调节效应,表明磷酸盐供应水平能够影响iATP和eATP的水平;NO对细胞ATP水平具有调节作用,其调节效应与环境磷酸盐供应水平有关.
BY-2 tobacco suspension cells were used as experimental materials to investigate the changes in the intracellular ATP(iATP)and extracellular ATP(eATP)levels under different levels of phosphate supply and the regulatory role of nitric oxide in this process.The results showed that the level of iATP increased with the increasing level of phosphate supply,which was followed by an increase of eATP level and decrease of the content of NO.Under a normal level of phosphate supply,the application of NO donor increased the levels of iATP and eATP,while the inhibitor of NO synthesis reduced that of iATP and eATP.Under the condition of either a lower or high level of phosphate supply,the effects of NO donor and inhibitor of NO synthesis on the iATP and eATP levels were different from those under a normal level of phosphate supply.These studies suggested that differences in the levels of phosphate supply could affect the levels of iATP and eATP in plant cells.NO could play a role in regulating the changes in iATP and eATP levels,but the regulatory effect of NO in this process was related to the level of phosphate supply.
作者
王娟娟
达晓伟
王馨
庞海龙
贾凌云
冯汉青
WANG Juan-juan;DA Xiao-wei;WANG Xin;PANG Hai-long;JIA Ling-yun;FENG Han-qing(College of Life Science,Northwest Normal University,Lanzhou 730070,China;New Rural Development Research Institute,Northwest Normal University,Lanzhou 730070,China)
出处
《兰州大学学报（自然科学版）》
CAS
CSCD
北大核心
2023年第3期346-350,共5页
Journal of Lanzhou University(Natural Sciences)
基金
国家自然科学基金项目(31870246)
甘肃省重点研发计划项目(22YF7NA119)
甘肃省科技重大专项(22ZD1NA001)
甘肃省引导科技创新发展专项(2019ZX-05)
西北师范大学新农村发展研究院开放基金。
