摘要
为获得优质、抗病的同源四倍体紫菜薹(Brassica campestris var.purpurea Bailey)新材料,使用0.2 g·L^(-1)秋水仙素溶液点滴二倍体子叶顶端分生组织,经形态和解剖学、细胞学鉴定筛选得到同源四倍体植株。通过对二倍体、四倍体植株农艺性状和营养品质分析发现:四倍体植株的株高极显著增高;开展度、叶片宽、叶柄长显著增加;叶片数极显著降低。营养品质方面,可溶性蛋白、花青苷含量显著增高;可溶性糖和纤维素含量极显著降低;硝态氮含量显著降低。光合特性分析发现,四倍体植株的净光合速率、胞间CO_(2)浓度、气孔导度、蒸腾速率均高于二倍体,表明四倍体更能适应强光。抗病性分析发现,四倍体受灰霉菌侵染后,菌斑面积小,颜色淡,具有更强的抗病性。
In order to create new germplasm resource of tetraploid purple tsai-tai(Brassica campestris var.purpurea Bailey),0.2 g·L^(-1) colchicine solution was used to drip the apical shoot of diploid.Then,autotetraploid plants were identified by morphology,anatomy and cytology methods.Through the analysis of agronomic characters,the plant height,spreading degree,leaf width and leaf stalk length of tetraploid plants increased significantly.However,the number of leaves of tetraploid significantly reduced.In nutritional quality,the content of soluble protein and anthocyanin in tetraploid plants increased significantly,while the content of soluble sugar,fibrin and nitric nitrogen in tetraploid plants significantly reduced compared with diploid.The analysis of photosynthetic characteristics showed that the net photosynthetic rate,interintracellular CO_(2) concentration,stomatal conductance and transpiration rate of tetraploid plant were higher than that ofdiploid,indicating that tetraploid plants were more adaptable to strong light.The analysis of disease resistance showed that the tetraploid plants had stronger disease resistance.
作者
张晨
李孟杰
杨晓雪
王美云
肖栋
王建军
侯喜林
胡君
刘同坤
ZHANG Chen;LI Mengjie;YANG Xiaoxue;WANG Meiyun;XIAO Dong;WANG Jianjun;HOUXilin;HU Jun;LIU Tongkun(State Key Laboratory of Crop Genetics and germplasm innovation,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;Nanjing Suman Plasma Engineering Research Institute of Agricultural Sciences,Nanjing 210095,China;Agricultural and Rural Bureau of Luoshe Town,Huishan District,Wuxi,Jiangsu 214187,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2023年第7期1419-1428,共10页
Acta Horticulturae Sinica
基金
国家自然科学基金面上项目(32072575)
江苏省种业揭榜挂帅项目(JBGS[2021]015)
江苏高校优势学科建设工程项目(PAPD)。
关键词
紫菜薹
同源四倍体
秋水仙素
光合特性
灰霉菌
Brassica campestris var.purpurea
autotetraploid
colchicine
photosynthetic characteristics
Botrytis cinerea
