摘要
目的:评估油莎豆对SD大鼠是否存在致畸作用。方法:以100只3月龄SPF级SD大鼠为试验动物,设置0(对照)、2、4、8 g/kg共4个油莎豆浓度,在SD大鼠孕期的第6~15天,灌胃给予受试物,每天1次。分别在受孕后第0、6、9、12、15、20天称量大鼠,于妊娠第20天采用CO_(2)麻醉法处死孕鼠,剖腹取出子宫,称取子宫连胎质量,记录并检查黄体数、着床数、吸收胎、早死胎、晚死胎及活胎数,活胎鼠记录胎仔体质量、体长,并检查胎鼠外观有无畸形。结果:各剂量组孕鼠在观察期内精神状态、一般活动未发现异常体征,第20天处死后大体解剖肉眼均未见明显异常;各分组试验数据统计结果显示,油莎豆对孕鼠一般情况、体质量及其增量、胚胎发育、胎鼠外观畸形、胎鼠骨骼发育、骨骼畸胎率、胎鼠内脏发育、畸胎率、孕鼠总畸胎率均无显著影响(P>0.05)。结论:在本实验条件下,油莎豆对SD大鼠无致畸作用。
OBJECTIVE:To assess whether there was a teratogenic risk from Cyperus esculentus on SPF grade pregnant SD rats.METHODS:For this study,1003-month-old SPF grade and pregnant rats were used.They were weighed on the 0^(th),6^(th),9^(th),12^(th),15^(th),and 20th days of gestation.On the 6th to 15th days of gestation,rats were fed at four concentration levels of Cyperus esculentus:0(CK),2,4,and 8 g/kg body weight.The pregnant rats were sacrificed using CO_(2) anesthesia on the 20th day of gestation.The uterus was taken out by laparotomy,and the weights of the uteri and fetuses were measured.The numbers of corpora lutea,implantation,absorption,early death,late death,and live fetus were recorded and examined.The fetal weights and body lengths were recorded for live fetal rats,and they were examined for abnormalities.RESULTS:For the pregnant rats,no abnormal physical signs were found,their general activities were normal,and no significant abnormalities were found in gross anatomy at the 20th day of execution.Results from analysis of the data show that Cyperus esculentus had no effect on the general situation,body weight and weight gain,embryonic development,fetal mouse appearance abnormalities,fetal mouse bone development,skeletal teratogenesis rate,fetal mouse visceral development,teratogenesis rate,and total teratogenesis rate of pregnant rats.CONCLUSION:Under our experimental conditions,there was no difference in abnormalities among the different exposure dose groups and from the controls.Thus,it can be determined that Cyperus esculentus did not cause teratogenic risk under our experimental conditions.
作者
李春鑫
于美琴
杨海龙
宋万献
张戈
王会伟
LI Chunxin;YU Meiqin;YANG Hailong;SONG Wanxian;ZHANG Ge;WANG Huiwei(Industrial Crops Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002;Zhengzhou Institute of Agriculture and Forestry Sciences,Zhengzhou 450005,Henan,China)
出处
《癌变·畸变·突变》
CAS
2023年第4期306-309,315,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
河南省重大科技专项(211100110100)
河南省农业科学院新兴学科发展专项(2023XK01)。
关键词
油莎豆
致畸性
SD大鼠
畸胎率
Cyperus esculentus
teratogenicity
SD pregnant rat
teratogenesis rate
