摘要
分别以美味牛肝菌的伞部和柄部为原料提取多酚氧化酶(PPO)。以伞部和柄部PPO酶活为指标,在单因素试验的基础上,通过响应面试验优化美味牛肝菌伞部和柄部PPO的提取工艺。结果表明:美味牛肝菌伞部PPO的最佳提取工艺为提取温度22℃、液料比2.0∶1(mL/g)、底物浓度1.0 mol/L、pH 7.4,在此条件下伞部PPO酶活为13.111 U;柄部PPO的最佳提取工艺为提取温度60℃、液料比2.2∶1(mL/g)、底物浓度0.5 mol/L、pH 5.9,在此条件下柄部PPO酶活为10.833 U。
Polyphenol oxidase(PPO)was extracted from the umbrella and stem of Boletus edulis Bull.:Fr.,respectively.Taking the activity of PPO in the umbrella and stem as index,the extraction process of PPO from the umbrella and stem of Boletus edulis Bull.:Fr.was optimized by response surface test on the basis of single factor test.The results showed that the optimal extraction process for PPO in the umbrella of Boletus eddulis Bull.:Fr.were as follows:extraction temperature 22,liquid-solid ratio 2.0:1(mL/g),substrate concentration 1.0 mol/L and pH 7.4.Under these conditions,the umbrella PPO activity was 13.111 U.The optimal extraction process of the stem PPO were as follows:extraction temperature 60℃,liquid-solid ratio 2.2:1(mL/g),substrate concentration 0.5 mol/L and pH 5.9.Under these conditions,the stem PPO activity was 10.833 U.
作者
白慧
陈若飞
阚欢
郭磊
BAI Hui;CHEN Ruo-fei;KAN Huan;GUO Lei(School of Life Science,Southwest Forestry University,Kunming 650224,Yunnan,China;Forestry Resources Exploitation and Utilization Engineering Research Center for Comprehensive Healthcare of Yunnan Provincial Universities,Southwest Forestry University,Kunming 650224,Yunnan,China)
出处
《粮食与油脂》
北大核心
2023年第7期138-141,共4页
Cereals & Oils
基金
云南省大学生创新训练项目(202110677011、202110677015)
云南省科技厅基础研究计划项目(202101AT070042)
国家重点研发计划重点专项项目(2019YFD1002404)。
关键词
美味牛肝菌
多酚氧化酶
提取工艺
响应面试验
Boletus edulis Bull.:Fr.
polyphenol oxidase
extraction process
response surface test
